Team:Paris/September 2

From 2008.igem.org

(Difference between revisions)
(→Ligation L173 (rbs-TetR in mRFP-LVA-tripart-pSB1A3))
(→Ligation L173 (rbs-TetR in mRFP-LVA-tripart-pSB1A3))
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|'''4:1''' (insert:vector) '''ratio'''
|'''4:1''' (insert:vector) '''ratio'''
|-
|-
-
|D112 (insert)
+
|'''D112''' (insert)
|0 µL
|0 µL
|1,4 µL
|1,4 µL
|1,9 µL
|1,9 µL
|-
|-
-
|D187 (vector)
+
|'''D187''' (vector)
|2,5 µL
|2,5 µL
|2,5 µL
|2,5 µL
|2,5 µL
|2,5 µL
|-
|-
-
|T4 DNA ligase 10X buffer
+
|T4 DNA ligase 10X '''buffer'''
|2 µL
|2 µL
|2 µL
|2 µL
|2 µL
|2 µL
|-
|-
-
|T4 DNA ligase
+
|T4 DNA '''ligase'''
|1 µL
|1 µL
|1 µL
|1 µL
|1 µL
|1 µL
|-
|-
-
|water
+
|'''water'''
|14,5 µL
|14,5 µL
|13,1 µL
|13,1 µL

Revision as of 16:24, 2 September 2008

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Ligation L173 (rbs-TetR in mRFP-LVA-tripart-pSB1A3)

Name Digestion number Digested by
rbs-TetR D112 EcoRI & SpeI (insert)
mRFP-LVA-tripart D187 EcoRI & XbaI (vector)


Control Sample
3:1 (insert:vector) ratio 4:1 (insert:vector) ratio
D112 (insert) 0 µL 1,4 µL 1,9 µL
D187 (vector) 2,5 µL 2,5 µL 2,5 µL
T4 DNA ligase 10X buffer 2 µL 2 µL 2 µL
T4 DNA ligase 1 µL 1 µL 1 µL
water 14,5 µL 13,1 µL 12,6 µL
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