Team:Paris/September 3

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(Difference between revisions)
(New page: {{Paris/Calendar_Links|September 2|September 4}} =Transformation of yesterday overnight (18 hours) ligation= Ligation '''L173''': *insert: '''D112''' (ES) rbs-TetR *vector: '''D187''' (E...)
(→Transformation of yesterday overnight (18 hours) ligation)
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{{Paris/Calendar_Links|September 2|September 4}}
{{Paris/Calendar_Links|September 2|September 4}}
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=Transformation of yesterday overnight (18 hours) ligation=
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=Construction of rbs-TetR-mRFP-LVA-tripart (L173)=
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[[Image:Part_icon_rbs.png]][[Image:icon_coding.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
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==Transformation ==
Ligation '''L173''':
Ligation '''L173''':

Revision as of 17:03, 16 September 2008

← Yesterday

↓ Calendar ↑

Tomorrow →

Construction of rbs-TetR-mRFP-LVA-tripart (L173)

Part icon rbs.pngIcon coding.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png

Transformation

Ligation L173:

  • insert: D112 (ES) rbs-TetR
  • vector: D187 (EX) mRFP-LVA-tripart

Insert:vector ratio : 3:1 or 4:1


Transformation of E. coli DH5 alpha competent cells

  • 100 µL of competent cells
  • 5 µL of DNA (ligation products or pUC19 for positive control)
  • 30 min on ice
  • heat shock 45 s at 42°C
  • 2 min on ice
  • 0,9 mL SOC
  • 1 hour at 37°C
  • plating on LB + ampicilline
  • overnight incubation at 37°C
Retrieved from "http://2008.igem.org/Team:Paris/September_3"