Team:Paris/September 4

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(Difference between revisions)
(Transformation results (rbs-TetR in mRFP-LVA-tripart-pSB1A3))
(Transformation results (rbs-TetR in mRFP-LVA-tripart-pSB1A3))
Line 27: Line 27:
-
L174 = d134 + d187 (pFlhB-mRFP LVA+)
+
L177 = d134 + d187 (pFlhB-mRFP LVA+)
 +
 
L175 = d149 + d° (pFliL - ECFP LVA+)
L175 = d149 + d° (pFliL - ECFP LVA+)
 +
L176 = d149 + d° (pFliL - ECFP LVA-)
L176 = d149 + d° (pFliL - ECFP LVA-)

Revision as of 19:24, 3 September 2008

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Transformation results (rbs-TetR in mRFP-LVA-tripart-pSB1A3)

Ligation L173

  • insert: D112 (ES) rbs-TetR
  • vector: D187 (EX) mRFP-LVA-tripart-pSB1A3
Sample positive control
pUC19
negative control
no DNA
ligation control
(without insert)
L173 (3:1 ratio) L173 (4:1 ratio)
Number of colonies


L177 = d134 + d187 (pFlhB-mRFP LVA+)

L175 = d149 + d° (pFliL - ECFP LVA+)

L176 = d149 + d° (pFliL - ECFP LVA-)