Team:Paris/September 5

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Purification of D112 (rbs-TetR)

Electrophoresis and gel extraction

D112 before gel excision

after gel excision

1% agarose gel

	Expected size	Measured size
pSB1A2 (vector)	2079 bp	2 kb
rbs-TetR (insert)	703 bp	0,7 kb

Qiaquick Gel Extraction Kit

Concentration measurement

Sample	DNA concentration	A260/A280
D112	8 µg/mL	2,31

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