Team:The University of Alberta/16 July 2008

From 2008.igem.org

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*The plates of Transformants of I0500 in the new vector turned out good
*The plates of Transformants of I0500 in the new vector turned out good
**Did colony PCR on two colonies to confirm they have the I0500 insert
**Did colony PCR on two colonies to confirm they have the I0500 insert
-
***Streaked out both colonies  
+
***Ran products out on a 1% agarose gel
-
***Also made O/Ns of the colonies
+
**Streaked out both colonies  
 +
**Also made O/Ns of the colonies
*Transformations of the synthed biobricks worked!
*Transformations of the synthed biobricks worked!
**Colony PCR to check the inserts
**Colony PCR to check the inserts
**Set up O/Ns
**Set up O/Ns

Latest revision as of 21:22, 16 July 2008

Today

  • The plates of Transformants of I0500 in the new vector turned out good
    • Did colony PCR on two colonies to confirm they have the I0500 insert
      • Ran products out on a 1% agarose gel
    • Streaked out both colonies
    • Also made O/Ns of the colonies
  • Transformations of the synthed biobricks worked!
    • Colony PCR to check the inserts
    • Set up O/Ns