"
Team:The University of Alberta/16 July 2008
From 2008.igem.org
(Difference between revisions)
(→Today) |
(→Today) |
||
| Line 3: | Line 3: | ||
**Did colony PCR on two colonies to confirm they have the I0500 insert | **Did colony PCR on two colonies to confirm they have the I0500 insert | ||
***Ran products out on a 1% agarose gel | ***Ran products out on a 1% agarose gel | ||
| - | + | **Streaked out both colonies | |
| - | + | **Also made O/Ns of the colonies | |
*Transformations of the synthed biobricks worked! | *Transformations of the synthed biobricks worked! | ||
**Colony PCR to check the inserts | **Colony PCR to check the inserts | ||
**Set up O/Ns | **Set up O/Ns | ||
Latest revision as of 21:22, 16 July 2008
Today
- The plates of Transformants of I0500 in the new vector turned out good
- Did colony PCR on two colonies to confirm they have the I0500 insert
- Ran products out on a 1% agarose gel
- Streaked out both colonies
- Also made O/Ns of the colonies
- Did colony PCR on two colonies to confirm they have the I0500 insert
- Transformations of the synthed biobricks worked!
- Colony PCR to check the inserts
- Set up O/Ns
