Team:The University of Alberta/18 July 2008

From 2008.igem.org

(Difference between revisions)
(New page: ===Today=== *Got the sequencing back that Winnie did on the BisDA gene: it was LLC because she used the primers; no big deal though because the size was correct and we're pretty sure that ...)
(Today)
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*Got the sequencing back that Winnie did on the BisDA gene: it was LLC because she used the primers; no big deal though because the size was correct and we're pretty sure that it's BisDA
*Got the sequencing back that Winnie did on the BisDA gene: it was LLC because she used the primers; no big deal though because the size was correct and we're pretty sure that it's BisDA
*Began westerns on the total/soluable crude butanol proteins
*Began westerns on the total/soluable crude butanol proteins
 +
*Digested Tryp and J6 with Xba and Pst.
 +
**Ligated them together, left the reaction to go overnight. Will transform tomorrow.

Revision as of 18:09, 18 July 2008

Today

  • Got the sequencing back that Winnie did on the BisDA gene: it was LLC because she used the primers; no big deal though because the size was correct and we're pretty sure that it's BisDA
  • Began westerns on the total/soluable crude butanol proteins
  • Digested Tryp and J6 with Xba and Pst.
    • Ligated them together, left the reaction to go overnight. Will transform tomorrow.