Team:The University of Alberta/27 May 2008

From 2008.igem.org

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NK 130-530
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'''Tom'''<ul>
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Transformation of the ligated I0500 vectors.
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Make amp plates when there is time.</ul>
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Hey guys, Kelly and I (Anthony) were in on Friday and we managed to excise the bands and do the gel purification.
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==Volunteers Scheduled for Today==
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Kelly has put Winnie's digested producs in the -20, I have put the purification products also in the -20 (wrapped together in green tape and labelled).
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As well, anything that was left out on the bench was put in the -20 because we have no clue what was in those tubes.
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Kelly and I were having a difficult time following the lab book, so PLEASE be explicitly clear about what has been performed, what is in each of the biobricks, etc... It might seem annoying and redundant but the volunteers have simply no way of following otherwise. A simple summary of each procedure and protocol would be GREATLY appreciated!
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We didn't have time to do to the sequencing reaction because Kelly has left for Rocky Mountain, and I am not about to start a 2.5 hr reaction at 7:30 PM on a Friday night!
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Good luck with things on Monday, and I will see you Tuesday
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10-3 BH<br>
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1-5:30 NK<br>
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4-8 AL<br>
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5-8 SG<br>
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6-8 DL
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'''Jason'''<ul>
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<li> Finished combining the edits for the fundraising letter.
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<li> Built Plant promoter and Resistance biobricks
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</ul>
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Anthony
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==Lab Tip of the Day==
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Growth chambers have settings for a reason (mainly so your plants don't die), so dont forget to make sure that you set your growth chamber properly. Going back the next day to water your plants only to find that all the lights shut off and the temperature is back down to 15 degrees can be depressing.

Latest revision as of 22:51, 27 May 2008

Tom
    Transformation of the ligated I0500 vectors. Make amp plates when there is time.

Volunteers Scheduled for Today

10-3 BH
1-5:30 NK
4-8 AL
5-8 SG
6-8 DL

Jason
  • Finished combining the edits for the fundraising letter.
  • Built Plant promoter and Resistance biobricks

Lab Tip of the Day

Growth chambers have settings for a reason (mainly so your plants don't die), so dont forget to make sure that you set your growth chamber properly. Going back the next day to water your plants only to find that all the lights shut off and the temperature is back down to 15 degrees can be depressing.