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Team:The University of Alberta/3 July 2008
From 2008.igem.org
(Difference between revisions)
(New page: ===Today=== '''Chris'''<br> *After staining the gels from yesterday, there were no apparent bands. Could the Ni-NTA column purifications not have worked? Will run two more gels, with the c...) |
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===Today=== | ===Today=== | ||
| + | Our primers finally arrived! We're still waiting on the site-directed mutageneis kit though (it's on back order) :( | ||
'''Chris'''<br> | '''Chris'''<br> | ||
*After staining the gels from yesterday, there were no apparent bands. Could the Ni-NTA column purifications not have worked? Will run two more gels, with the crude and uninduced purified samples as well as induced pure. | *After staining the gels from yesterday, there were no apparent bands. Could the Ni-NTA column purifications not have worked? Will run two more gels, with the crude and uninduced purified samples as well as induced pure. | ||
| + | **'''Ran the gels in the following order: | ||
| + | ***Gel 1: The I0500 parts (crude uninduced, purified uninduced, purified induced) | ||
| + | ***Gel 2: The J61003 parts (crude uninduced, pure (un)induced) | ||
'''Jason'''<br> | '''Jason'''<br> | ||
*Gel purified PCR products from the colony PCR yesterday | *Gel purified PCR products from the colony PCR yesterday | ||
| - | *Sequenced the purified products | + | *Sequenced the purified products - won't get the results back until Monday. |
'''Saima'''<br> | '''Saima'''<br> | ||
*Troubleshooting: tried to determine the source of the strange bands from yesterday's colony PCR (esp. the bands in the water controls) | *Troubleshooting: tried to determine the source of the strange bands from yesterday's colony PCR (esp. the bands in the water controls) | ||
'''Tom''' | '''Tom''' | ||
*Made two SDS-PAGE gels for Chris to use (see above) | *Made two SDS-PAGE gels for Chris to use (see above) | ||
Latest revision as of 21:03, 3 July 2008
Today
Our primers finally arrived! We're still waiting on the site-directed mutageneis kit though (it's on back order) :(
Chris
- After staining the gels from yesterday, there were no apparent bands. Could the Ni-NTA column purifications not have worked? Will run two more gels, with the crude and uninduced purified samples as well as induced pure.
- Ran the gels in the following order:
- Gel 1: The I0500 parts (crude uninduced, purified uninduced, purified induced)
- Gel 2: The J61003 parts (crude uninduced, pure (un)induced)
- Ran the gels in the following order:
Jason
- Gel purified PCR products from the colony PCR yesterday
- Sequenced the purified products - won't get the results back until Monday.
Saima
- Troubleshooting: tried to determine the source of the strange bands from yesterday's colony PCR (esp. the bands in the water controls)
Tom
- Made two SDS-PAGE gels for Chris to use (see above)
