Team:The University of Alberta/3 July 2008

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Today

Chris

After staining the gels from yesterday, there were no apparent bands. Could the Ni-NTA column purifications not have worked? Will run two more gels, with the crude and uninduced purified samples as well as induced pure.

Jason

Gel purified PCR products from the colony PCR yesterday
Sequenced the purified products

Saima

Troubleshooting: tried to determine the source of the strange bands from yesterday's colony PCR (esp. the bands in the water controls)

Tom

Made two SDS-PAGE gels for Chris to use (see above)

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