Alberta NINT/3 July 2008
From 2008.igem.org
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lab work (SD):
Sequenced pSB4A5 to verify. Made overnights from four colonies from each plate (K102022 and K102032) from 02/07/08.
lab work (JD):
From sequencing K102006.4 becomes official K10200 and K102007.5 becomes official K102007 Digested pSB4A5 with EcoR1 and Pst1 Digested K102006 and K102003 with EcoR1 and Pst1 Ran 2% agarose gel electrophoresis Made glycerol stock of K102006 and K102007