Brown: Team Resistance/11 July 2008

From 2008.igem.org


Toxipop lab notebook.png

11 July 2008

Ran restriction digest of both pDKL02 (IPTG-induced promoter) and pVJ4 at the EcoRI and EcoRV sites (should cut halfway through the cassette)

  • Mini-prepped the pDKL02 DNA
  • Only saw band for pDKL02 but assumed that it must also be there in the pVJ4 but something went wrong

This week we did a lot of work with the apparatus because it was knocked over and disconnected and when reconnected, it did not give consistent readings at all

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