25 September 2008
- Growth seen in 3:1 and 6:1 ligations!
- Ligation cultures miniprepped.
- 3:1 Restriction Digest (X2)
- 1 ul EcoRI
- 1 ul HindIII
- 5 ul Buffer E
- .5 ul BSA
- 10 ul DNA
- 32.5 ul sterilized water
- 6:1 Restriction Digest (X2)
- 1 ul EcoRI
- 1 ul HindIII
- 5 ul Buffer E
- .5 ul BSA
- 9 ul DNA
- 33.5 ul sterilized water
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