Brown: Team Resistance/28 October 2008

From 2008.igem.org


Toxipop lab notebook.png

28 October 2008

  • Performed minipreps on pS105 and pVJ4.
  • Optical Densities:
    * pVJ4 2 - 73.2 ng/ul
    * pVJ4 3 - 116.6 ng/ul
    * pS105 1 - 77.2 ng/ul
    * pS105 3 - 107.5 ng/ul
  • Minipreps were also done of multiple promoters from the Registry of Standard Biological Parts.
  1. BBa_I728456 - Mercury Inducible Promoter MerRT
  2. BBa_I0500 - Inducible pBAD/araC
  3. BBa_I712074 - T7 promoter (strong promoter from T7 bacteriophage)
  4. BBa_I723020 - Pu: Activated in presence of environmental xylene.
  5. BBa_R0010 - Promoter (lacI regulated)
  • Conductance test ran yesterday. Lysis took a considerably long time. Left overnight. The cultures with added Arabinose cleared considerably more than the controls.