Brown: Team Resistance/7 June 2008

From 2008.igem.org


Toxipop lab notebook.png

June 7, 2008

Resistance Apparatus Design

We needed to create an apparatus that could hold multiple plates so that multiple controls and experiments could be run in parallel:

5 across

  1. culture alone
  2. lb alone
  3. culture + reagent
  4. lb + reagent
  5. previously lysed cells+ reagent

3 down (3 trials of each solution)

Constructed 3 layer wall of legos around the 15 plates with arms in each corner of the wall to support the cardboard which will hold the wires in place

frame: 13.75 in. x 9.00 in. (3 sides) top: 28.8 cm x 19.1 cm

wires slipped through cardboard distance between each wire in plates 0.25 in. and 1.73 in. into plates