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• Team:BrownTwo/Notebook
  ...er, and 1.5 uL T4 DNA ligase. Decrease water if you use higher volumes of insert and vector. Maintain total volume of 15 uL. </p> ...me volume of insert and vector. If one uses a volume ratio of 3:1 between insert and vector, the ratio of DNA amounts will end up being 10.494, which is eve
  226 KB (32,672 words) - 04:53, 29 October 2008
• Team:Johns Hopkins/Notebook
  [Make general comments here, so they don't get lost in peoples e-mail boxes] == Data ==
  11 KB (1,649 words) - 04:50, 30 October 2008
• Jamboree/Project Abstract/Team Abstracts
  ...e Program [https://static.igem.org/mediawiki/2008/6/6c/Igem_08_program.pdf here].</span> In our project, we designed a novel device by which we could insert different unnatural amino acids into a certain site in target protein expre
  76 KB (11,186 words) - 15:27, 6 November 2008
• Team:Johns Hopkins/Notebook/GROUP 3: Short two way stops
  and cutting with Nsp1. The data is somewhat hard to decipher, but I am working on it... Each Fluorescent Protein had no insert present. However NEB buffer 2 was used and >5 ug of DNA was used.
  7 KB (1,012 words) - 01:06, 29 October 2008
• Team:Paris/Modeling/BOB
  * The goal here is to present the differential equations we used in the model. At each step * Here is an overall view of the whole system :
  24 KB (3,732 words) - 04:46, 30 October 2008
• Team:LCG-UNAM-Mexico/Notebook/2008-August 2
  <p>*In order to verify the size of the insert in pBB</p> ...from the Mexico-UNAM-IPN with several questions in regards to our project, here is our reply:<br />
  53 KB (7,909 words) - 00:14, 30 October 2008
• Team:BCCS-Bristol/Notebook
  ...for T9002 and E0840 were as expected and corresponded to the part sequence data. ...as used in both the single and double digest and according to the sequence data cuts once in the plasmid containing the J37015 part (pSB1A2). HindIII was
  32 KB (4,961 words) - 22:30, 28 October 2008
• Team:Edinburgh/Results/Bacillobricks
  ...ly demonstrated that this reporter gene works well in ''B. subtilis''. The insert was cut out with EcoRI and PstI and ligated with pTG262 cut with the same t ...size), it was expected that half of the colonies would contain the correct insert. In fact, one of four clones tested showed evidence of XylE activity (ie, a
  12 KB (1,964 words) - 02:11, 30 October 2008
• Team:Edinburgh/Protocols/Bacillobricks
  ...ly demonstrated that this reporter gene works well in ''B. subtilis''. The insert was cut out with EcoRI and PstI and ligated with pTG262 cut with the same t ...size), it was expected that half of the colonies would contain the correct insert. In fact, one of four clones tested showed evidence of XylE activity (ie, a
  12 KB (1,964 words) - 02:21, 30 October 2008
• Imperial College/20 August 2008
  All major bands correspond to the approximate size of vector and insert. In several of the minis a second band is however visible is most likely to ...ype/Wetlab/Transformation_protocol_4#Transformation_Protocol_2| Click link here for protocol] was carried out on competent cells that had been grown to an
  2 KB (337 words) - 20:38, 28 October 2008