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From 2008.igem.org

Create the page "Standard Media" on this wiki!

  • Main Page
    ...boree? See the <a class="pink" href="http://2008.igem.org/Jamboree/Media">Media</a> page for pictures and videos. Post your own links to let us know where **[[Jamboree/Media | Media ]]<small>(pictures & fun videos!)</small>
    10 KB (1,324 words) - 20:30, 5 May 2016
  • Jamboree/Compete
    ...mboree/Compete#Requests_for_variance">Submit any requests for non-BioBrick standard methods</a> : <span class="deadline">October 8</span></li> ...xpected to submit their parts to the [http://partsregistry.org Registry of Standard Biological Parts]. In addition to submitting your parts, you must also dec
    13 KB (1,920 words) - 06:14, 22 April 2016
  • Teachtheteachers
    <b>10:00 am -- Jason Kelly -- Standard Measurement Kit for BioBrick Promoters (PoPS-out devices)</b><br> ...xplains how to measure the PoPs of promoters and compare it to a reference standard.
    5 KB (778 words) - 20:34, 8 May 2008
  • Team:Alberta NINT/Project
    [[Media:NINT_PlasmidDesignAndConstruction.pdf|Plasmid Design and Construction]] ...ion in the absence of any input. The y-axis is shown in Miller units (the standard for such assays).
    21 KB (3,119 words) - 16:44, 30 March 2009
  • Team:Hawaii/Make Competent E. Coli
    ...sse '464 patent]] describes using this buffer for DH5&alpha; cells. The [[Media:pat6709852.pdf | Bloom04]] patent describes the use of essentially the same ...ed by [[User:Tk|Tom Knight]] and the [http://partsregistry.org Registry of Standard Biological Parts].
    7 KB (1,158 words) - 23:54, 30 May 2008
  • SOB
    [[Category:Material]] [[Category:Standard Media]]
    1 KB (171 words) - 02:13, 31 May 2008
  • SOC
    #Follow directions to make 1 liter of [[SOB]] media [[Category:Material]] [[Category:Standard Media]]
    546 B (73 words) - 02:24, 30 May 2008
  • Team:Hawaii/Member Training
    ## Read [[Image:Idempotent Vector Design for Standard Assembly of Biobricks.pdf|Biobrick Technical Manual]], don't worry about un ## [http://igem.blip.tv/file/254379 Drew Endy: What is a Standard Biological Part] 2 minute 31 seconds
    2 KB (309 words) - 12:16, 31 May 2008
  • Team:Purdue/Project
    ...lacZ'' has been used for blue/white screening of ''E. coli'' for decades. Standard ''E. coli'' naturally make the lacZ protein (beta-galactosidase, or beta-ga ...ect one. For this project, we are using the ''recA'' promoter, which is a standard promoter of the SOS pathway and is activated only after extreme DNA damage.
    12 KB (1,859 words) - 23:49, 29 October 2008
  • Team:Illinois/Antibody RTK Fusion
    ...pplies needed will include the cholera B subunit, yeast culture media, and standard molecular biology reagents/kits.
    7 KB (1,026 words) - 04:36, 30 October 2008
  • Team:Illinois/Antibody GPCR Fusion
    ...of the FUS1-Fluo.Prot. fusion into the yeast genome, which seems to be the standard for this type of thing. *Preparation of Yeast Media [http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/article/m
    21 KB (3,227 words) - 02:19, 24 October 2008
  • Team:Hawaii/Project/Part B
    ...A'' and ''slr2016'' secretion signal sequences will be syntheized with the standard Biobrick sites. Oligonucleotide fragments of each will be hybridized with i ...efficacy of the signal peptides in transporting GFP into the extracellular media can be measured using a spectrofluorometer.
    11 KB (1,558 words) - 02:25, 29 October 2008
  • Team:UC Berkeley/Protocols
    # Plate using the sterilized glass-bead method or with standard spreaders. # Plate using the sterilized glass-bead method or with standard spreaders.
    17 KB (2,857 words) - 22:03, 27 October 2008
  • Team:Hawaii/Protocols/Competent Cells
    ...sse '464 patent]] describes using this buffer for DH5&alpha; cells. The [[Media:pat6709852.pdf | Bloom04]] patent describes the use of essentially the same ...ed by [[User:Tk|Tom Knight]] and the [http://partsregistry.org Registry of Standard Biological Parts].
    5 KB (802 words) - 04:22, 24 June 2008
  • Team:University of Chicago/Notebook/Norayucel
    7. Added 250microliters SOC media (prepared at 1:30)<br> ...0microliters for pGreen, 1mL for E0240 (scaled up to accommodate for extra media dilution)
    11 KB (1,571 words) - 16:42, 28 September 2008
  • Team:University of Washington/Project
    ...ry that provided a PoPS output when these three conditions are met. Using standard BioBrick assembly methods, we placed the gene for LuxR under the control of ...design of projects like ours easier using information from the Registry of Standard Biological Parts (see SeToB tab).
    23 KB (3,437 words) - 04:40, 30 October 2008
  • Jamboree/Project Abstract/Team Abstracts
    ...rupt. We also produced a graphical interface for exploring the Registry of Standard Biological Parts called Viz-A-Brick (http://gcat.davidson.edu/VizABrick/), ...h the following specifications: the promoters must conform to the BioBrick standard; they must be modular so they can be used multiply in devices; and they mus
    76 KB (11,186 words) - 15:27, 6 November 2008
  • Team:Waterloo/Project
    ...nucleases during uninduced conditions. Cultures will initially be grown on media containing IPTG. Expression of the unstable cI repressor (C0051) will be dr ...me degradation construct will be integrated into the genome via a proposed standard integration vector.
    11 KB (1,711 words) - 04:00, 30 October 2008
  • Team:Groningen/Notebook
    ...e [http://partsregistry.org/Assembly:Standard_assembly <font color=#dd0000>Standard Assembly from the parts registry</font color>] site. When we had to assembl '''Media'''
    4 KB (643 words) - 12:10, 16 September 2008
  • Team:Waterloo/Notebook
    ...plication written in-house for administration of BioBricks. It understands standard assembly and can gather information from the Parts Registry. BioMortar will ...hat it can construct sequences for composite parts automatically. Although Standard Assembly makes it easy to ligate parts, it is fairly easy to reverse the en
    3 KB (456 words) - 04:05, 30 October 2008

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