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  • Team:Heidelberg/Notebook/Killing II/10thweek
    ...CR-Screen: Colonies 1, 2, 3, 4, 7 & 8 positive; Inoculation of 5 ml LB-Amp media with this colonies for miniprep, 37 °C -> ON *Miniprep of pSB1A2-Receiver-ColE9lys (BioBrick Standard): colonies 1, 2, 3, 4, 7 & 8, eluted in 35 µl H<sub>2</sub>O
    43 KB (6,079 words) - 21:44, 28 October 2008
  • Team:Caltech/Project/Vitamins via the addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG)to the media. This will allow us to test overexpression of each plasmid separately. In a ...haracterized using known quantities of folic acid in assay media. Once the standard curve has been established, then experimental growth levels, as quantified
    23 KB (3,375 words) - 18:48, 20 October 2008
  • Team:Heidelberg/Notebook/material
    ...gonucleotides were purchased from Invitrogen (Karlsruhe) and adjusted to a standard concentration of 100 <sup>pmol</sup>/<sub>µl</sub>. ===Bacteria Growth Media===
    69 KB (9,346 words) - 09:15, 20 August 2009
  • Team:Caltech/Protocols/Folate assay
    ...and sample tubes to light. I usually wrapped the buffers, assay media, and standard curve folic acid dilutions in aluminum foil. I also started covering the en ...ctobacilli Broth AOAC is recommended for ''L. rhamnosus'', so we used this media in place of the recommended Lactobacilli MRS broth.
    6 KB (840 words) - 00:48, 30 October 2008
  • Team:Heidelberg/Human Practice/Essay
    ...ut it. Furthermore we wanted to ask scientists about there opinion towards media contacts and the publication of their work for a broad audience. This knowl are positive: More scientists feel that they benefit from work with the media and popularizing their work than it would have been assumed.[[Team:Heidelbe
    41 KB (6,314 words) - 15:28, 29 October 2008
  • Team:Calgary Wetware/Protocols
    ...e foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want</li> <li>Weigh 35g of LB-Agar powder mix per litre of media desired. One litre makes 40-50 plates</li>
    23 KB (3,681 words) - 23:18, 29 October 2008
  • GenProtocols
    #Grow 5mL culture of bacteria in LB media overnight, 37° C , shaking #Remove media and wash with 0.33 volumes (330μL) of 1M sorbitol
    17 KB (2,715 words) - 19:24, 26 October 2008
  • Jamboree/Project Abstract/Team Abstracts
    ...rupt. We also produced a graphical interface for exploring the Registry of Standard Biological Parts called Viz-A-Brick (, ...h the following specifications: the promoters must conform to the BioBrick standard; they must be modular so they can be used multiply in devices; and they mus
    76 KB (11,186 words) - 15:27, 6 November 2008
  • Team:Cambridge/Improved GFP
    ...the only GFP variant currently listed and characterised in the Registry of Standard Biological Parts is the somewhat outdated GFP mut3b [3] . <br> ...anced versions of GFP – in standard BioBrick format - to the Registry of Standard Biological Parts would thus be expected to have a range of positive effects
    12 KB (1,879 words) - 03:48, 30 October 2008
  • Team:Hawaii/Make Competent E. Coli
    ...sse '464 patent]] describes using this buffer for DH5&alpha; cells. The [[Media:pat6709852.pdf | Bloom04]] patent describes the use of essentially the same ...ed by [[User:Tk|Tom Knight]] and the [ Registry of Standard Biological Parts].
    7 KB (1,158 words) - 23:54, 30 May 2008
  • Team:Hawaii/Protocols/Competent Cells
    ...sse '464 patent]] describes using this buffer for DH5&alpha; cells. The [[Media:pat6709852.pdf | Bloom04]] patent describes the use of essentially the same ...ed by [[User:Tk|Tom Knight]] and the [ Registry of Standard Biological Parts].
    5 KB (802 words) - 04:22, 24 June 2008
  • Team:IIT Madras/Project an essential part of the synthetic biologist's toolkit. The Registry of Standard Biological Parts contains a growing number of promoters whose expression ca ...ements. We are currently characterizing the library of promoters against a standard control, the unmodified Lutz-Bujard LacO promoter.
    7 KB (1,125 words) - 14:42, 27 October 2008
  • Team:Caltech/Project/Oxidative Burst
    The constructs shown in figure 5 were made using the standard assembly method. They were made to test the various regulatory elements and ... arrow indicates induction with AHL at time 0 hr. Error bars represent one standard deviation(n=3) and are too small to be visible on the negative control.]]
    16 KB (2,564 words) - 20:23, 25 October 2008
  • Team:Heidelberg/Project/Killing II
    ...sually are not secreted, whereas colicins of group A are released into the media.[[Team:Heidelberg/Project/Killing_II#References|[4]]], [[Team:Heidelberg/Pr ...ot elucidated so far. Clear is that most of the colicin is released in the media, but only in a late state of release small amounts of lysis proteins were d
    65 KB (9,654 words) - 17:16, 29 October 2008
  • Team:Purdue/Project
    ...lacZ'' has been used for blue/white screening of ''E. coli'' for decades. Standard ''E. coli'' naturally make the lacZ protein (beta-galactosidase, or beta-ga ...ect one. For this project, we are using the ''recA'' promoter, which is a standard promoter of the SOS pathway and is activated only after extreme DNA damage.
    12 KB (1,859 words) - 23:49, 29 October 2008
  • Team:Harvard/Dailybook/Week1/Chemical and Light
    None of the samples in the LB amp media grew. All the others grew and then were miniprepped and stored in glycerol 6/26: Amp cultures still did not grow in liquid media, neither at 1:1000 or a 1:2000 dilution of amp
    34 KB (5,237 words) - 18:54, 26 October 2008
  • Team:Harvard/Dailybook/Week4/Chemical and Light
    8 100μL PCR reactions set up (standard mix): DNA template is S1 P13, 40 cycles, annealing temp is 58°C, extension 8 100μL PCR reactions set up (standard colony mix) w/ WT Shewanella
    38 KB (5,639 words) - 19:03, 26 October 2008
  • Minnesota/23 September 2008
    ...d fragment was cleaned by ethanol precipitation. The size of the reference standard promoter is 35 bp. (was obtained from source plate 1002, well number 10F). ...late (to preserve the individual clones) and inoculated separately into LB media to do plasmid isolation.
    3 KB (487 words) - 20:11, 29 October 2008
  • Team:Bologna/Biosafety
    ...terns in the building. Work is generally conducted on open benchtops using standard practices. Usually, "contaminated" materials are left in open (but separate # the recommended Standard Microbiological Practices, Special Practices, and Safety Equipment for BSL3
    29 KB (4,412 words) - 02:19, 30 October 2008
  • Materials and Methods
    Yeast were transformed using standard procedures (LiOAc method). ...gal2::NatR, allowing graded activation of galactose inducible promoters ([[Media:Hawkins and Smolke.pdf]]).
    3 KB (508 words) - 23:50, 29 October 2008

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