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Create the page "Team:Hawaii/Protocol" on this wiki!

Team:Hawaii/Birds Eye View
{{Team:Hawaii/Common.css}} {{Team:Hawaii/Header}}

5 KB (626 words) - 03:27, 30 October 2008
Team:Hawaii/Project
{{Team:Hawaii/Header}} ===<onlyinclude>[[Team:Hawaii/Project/Part A|Part A]]: Mobilizable Broad-Host-Range Plasmid </onlyinclude

7 KB (1,011 words) - 03:46, 30 October 2008
Team:Hawaii/Construction of Broad-Host-Range Expression Vector
{{Team:Hawaii/Header}} ===[[Team:Hawaii/PCR Amplification of pRL1383a|PCR Amplification of pRL1383a]]===

5 KB (667 words) - 01:10, 16 August 2008
Team:Hawaii/Initial Synth. Oligo Assembly
==Protocol== ===[[Team:Hawaii/Protocols/gel_extraction|Gel extraction]]===

19 KB (3,019 words) - 16:40, 25 July 2008
Team:Hawaii/PCR Amplification of pRL1383a
{{Team:Hawaii/Header}} * 1uL pRL1383a as template (1:10 dilution of [[Team:Hawaii/Large-Scale Preparation of Plasmid from E. coli|large-scale plasmid prep]])

22 KB (3,349 words) - 00:48, 30 October 2008
Team:Hawaii/PCC6803 Electroporation of PCC6803
{{Team:Hawaii/Header}} **A [[Team:Hawaii/PCC6803 cell density| PCC6803 cell density]] test was performed and aligne

3 KB (464 words) - 18:33, 30 June 2008
Team:Hawaii/Notebook/2008-06- 2
{{Team:Hawaii/Header}} :* Protocol suggests 23C but growth was too slow. Also, protocol is for Mach10 cells, which grows much faster.

3 KB (445 words) - 02:35, 5 June 2008
Team:Hawaii/Notebook/2008-07-16
{{Team:Hawaii/Header}} :*pSB3K3 was restreaked [[Team:Hawaii/Notebook/2008-07-15|yesterday]], and today I am growing them up in LB media

1 KB (183 words) - 19:57, 17 July 2008
Team:Hawaii/Milestone
{{Team:Hawaii/Header}}[[Image:20080612-work breakdown structure.jpg|thumb|300px|right]] * Culturing Protocol Optimization: CO2 is bottle neck, need incubator retrofitted (KS)

2 KB (219 words) - 17:35, 15 August 2008
Team:Hawaii/Notebook/2008-07-22
{{Team:Hawaii/Header}} ...number. Used the protocol from Dr. Callahan's lab ([[http://packrat.stjohn.hawaii.edu/prestinglab/wiki/Protocols]]).

3 KB (438 words) - 00:40, 26 July 2008
Team:Hawaii/Large-Scale Preparation of Plasmid from E. coli
{{Team:Hawaii/Header}} ...can be obtained by using CsCl/ethidium bromide equilibrium centrifugation (protocol found in Maniatis).

10 KB (1,527 words) - 08:31, 10 July 2008
Team:Hawaii/Meeting/2008-06-12
{{Team:Hawaii/Header}} '''Krystle: Signal Peptides''' [[Team:Hawaii/Project/Part_B | (see project proposal)]]

3 KB (536 words) - 21:46, 12 June 2008
Team:Hawaii/Test Competent E. Coli
...onential growth of E. coli seed stocks at 23C (Callahan cells, recommended protocol) result in higher competency than growth of E. coli at 37C (our cells)? ... cells were made on 7/11/08 with this [[Team:Hawaii/Make Competent E. Coli|protocol]].

4 KB (614 words) - 01:33, 16 July 2008
Team:Hawaii/Notebook/2008-06-17
{{Team:Hawaii/Header}} :* Same protocol as 6/3/08 competency test

1 KB (183 words) - 04:31, 18 June 2008
Team:Hawaii/Notebook/2008-06-20
{{Team:Hawaii/Header}} ===Electroporation Protocol===

705 B (98 words) - 06:51, 24 June 2008
Team:Hawaii/Notebook/2008-06-10
{{Team:Hawaii/Header}} ...r comparison of transformation efficiency with our competent cells. [[Team:Hawaii/Biobricks_extraction_%26_transformation_competency_test|(see experiment)]]

2 KB (225 words) - 01:06, 12 June 2008
Team:Hawaii/Notebook/2008-07- 9
{{Team:Hawaii/Header}} :* Followed protocol used for transformation of cells with BioBrick plasmids from filter

1 KB (167 words) - 03:41, 15 July 2008
Team:Hawaii/Meeting/2008-07-10
{{Team:Hawaii/Header}} # Oligo Annealing Ligation Protocol Updates(GK, KS)

766 B (106 words) - 08:18, 10 July 2008
Team:Hawaii/Notebook/2008-07-10
{{Team:Hawaii/Header}} ...) and annealed but not ligated products. Gel still had ladders. See [[Team:Hawaii/Initial_Synth._Oligo_Assembly|experiment]].

2 KB (273 words) - 09:10, 14 July 2008
Team:Hawaii/Project/Part B MaterialsMethodsResults
...es were created. The first followed ligation and restriction digest [[Team:Hawaii/Protocols |protocols]] using full concentrations of annealed products. The Following standard PCR protocol, [http://www.partsregistry.org/Part:BBa_E0040 GFP] was converted into the f

13 KB (1,867 words) - 03:04, 30 October 2008