Team:Hawaii/Notebook/2008-06-17

From 2008.igem.org

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Things we did today

Wetlab work

Tested competency of new batch of competent cells

Grace
  • Same protocol as 6/3/08 competency test
  • Plated 20 μl untransformed competent cells on LB+amp100 as negative control

Biobricks extraction

Grace
  • Extracted BBa_B0024, BBa_B0025, and BBa_E0240 from filter paper using same protocol 6/9/08

Alkaline lysis

Grace
  • Made stock solutions
  • 0.5M EDTA
  • 1M Tris-HCl (pH 8.0)
  • 5M KOAc (3M potassium, 5M acetate, pH 4.8)
  • Made alkaline lysis solutions
  • GTE (50 mM glucose, 25 mM Tris-HCl, 10 mM EDTA)
  • 0.2M NaOH/1% SDS

Made TES buffer

Norman

Drylab Work

Primers

Norman
  • Refined primers

Discussion

  • Plasmid has not dried yet (ethanol still evaporating) so it will dry in the hood overnight. Norman will resuspend the pellet in 30 μl TE buffer tomorrow morning.

Quote of the Day

"You could have had a Nature publication, you know." - Dr. Presting, in reference to a cytochrome article and Grace's refusal to work with the pigments


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