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Team:Hawaii/Notebook/2008-06-17
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Tested competency of new batch of competent cells
- Grace
- Same protocol as 6/3/08 competency test
- Plated 20 μl untransformed competent cells on LB+amp100 as negative control
Biobricks extraction
- Grace
- Extracted BBa_B0024, BBa_B0025, and BBa_E0240 from filter paper using same protocol 6/9/08
Alkaline lysis
- Grace
- Made stock solutions
- 0.5M EDTA
- 1M Tris-HCl (pH 8.0)
- 5M KOAc (3M potassium, 5M acetate, pH 4.8)
- Made alkaline lysis solutions
- GTE (50 mM glucose, 25 mM Tris-HCl, 10 mM EDTA)
- 0.2M NaOH/1% SDS
- Extracted pRL1383a from transformed cells (see experiment)
Made TES buffer
- Norman
Drylab Work
Primers
- Norman
- Refined primers
Discussion
- Plasmid has not dried yet (ethanol still evaporating) so it will dry in the hood overnight. Norman will resuspend the pellet in 30 μl TE buffer tomorrow morning.
Quote of the Day
"You could have had a Nature publication, you know." - Dr. Presting, in reference to a cytochrome article and Grace's refusal to work with the pigments
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
