Team:Cambridge/Signalling/Experiments/AHL degrade

From 2008.igem.org

Home Voltage Signalling Bacillus Modelling Protocols People

Signalling Overview Vectors Constructs Primers Experiments Lab Notebook

x

Testing different volumes of AHL

Our previous experiments were inconclusive because we believed that we possibly swamped our Bacillus with excess amounts of AHL so that no appreciable degradation was seen. In this experiment we tested and compared different concentrations of AHL.

Fluorescence with 1 uM AHL

Without Bacillus: 2457

With Bacillus: 2497

Fluorescence with 10nM AHL

Without Bacillus: 1424

With Bacillus: 1318

Fluorescence with 1nM AHL

not enough for good readings

Images of 1uM AHL plates with accompanying plot profiles

The graphs demonstrate fluorescence change across the plate from left to right.

AHL without Bacillus

AHL with Bacillus

x