Team:Chiba/Calendar-Home/4 September 2008

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3 September 2008 <|> 5 September 2008

Contents

1 Laboratory work

Laboratory work

Team:Input

no work

Team:Communication

--->Gel Check

Sample DNA	1
Loading Dye	1
dH₂O	4
TOTAL	6μL

From left;

[http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2007) -> OK

[http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2007) -> OK

[http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2006) -> OK

[http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2006) -> OK

From left;

[http://partsregistry.org/Part:BBa_C0161 BBa_C0261](2007) -> OK

[http://partsregistry.org/Part:BBa_C0161 BBa_C0261](2007) -> OK

[http://partsregistry.org/Part:BBa_C0161 BBa_C0261](2006) -> OK

[http://partsregistry.org/Part:BBa_C0161 BBa_C0261](2006) -> OK

--->Digestion test

Sample	Single Digesiton	Double Digestion
Sample DNA	1	2
EcoRI	0.05	0.05
PstI	-	0.05
Buffer 3	1	1
BSA	1	1
dH₂O	6.95	5.9
TOTAL	10μL	10μL

--->Gel Check

Sample DNA	10
Loading Dye	2
TOTAL	12μL

From left;

Single Digestion

[http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2007)
[http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2007)
[http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2006)
[http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2006)
[http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2007)
[http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2007)
[http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2006)
[http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2006)

ladder

Double digestion

[http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2007)
[http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2007)
[http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2006)
[http://partsregistry.org/Part:BBa_C0161 BBa_C0161](2006)
[http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2007)
[http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2007)
[http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2006)
[http://partsregistry.org/Part:BBa_C0261 BBa_C0261](2006)

Transformation

Competent cells : JW1908

Transformed the following and grew on new ampicillin plates.

[http://partsregistry.org/Part:BBa_K084009 BBa_K084009](R-3,4,6,7)
[http://partsregistry.org/Part:BBa_K084010 BBa_K084010](C-1,2,6,7)
[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)

--->(5/9)Liquid Culture

Team:Output

[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] x3
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003] x3

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