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Team:Chiba/jk/β/weak 5
From 2008.igem.org
>送受信班
Contents |
Week 5
>last week
14 September, 2008
- (13/9)--->Mini prep
- Transformation
- Competent cells : XL10G
- BBa_T9002[http://partsregistry.org/Part:BBa_T9002](2007)
- Plac+RhlI+LVA
- Plac+CinI+LVA
- --->(15/9)
15 September, 2008
- Transformation
- competent cells : BW⊿FliC
- BBa_S03623 [http://partsregistry.org/Part:BBa_S03623]
- BBa_K084007 [http://partsregistry.org/Part:BBa_K084007]
- BBa_K084008 [http://partsregistry.org/Part:BBa_K084008]
- BBa_K084009 [http://partsregistry.org/Part:BBa_K084009]
- BBa_T9002 [http://partsregistry.org/Part:BBa_T9002]
(15/9)->cultured in new LB-Amp medium.
added AHL(total concentration was 0nM,1nM,10nM,100nM,1000nM)
- -->check green fluorescence intensity(Excitation:485nm,Emission:527nm)
Cultured transformants(LB,37℃,14h)
- BBa_K084007~BBa_K084009
- BBa_T9002
- BBa_S03623
cultured above in new LB medium.
-->mix(16/9)
16 September, 2008
(15/9)->mix cultures in the way prescribed in the Table below.
- -->check green fluorescence intensity(Excitation:485nm,Emission:527nm)
17 September, 2008
18 September, 2008
19 September, 2008
20 September, 2008
>next week
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