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Team:Harvard/Dailybook/Week5/Widgetry
From 2008.igem.org
Contents |
Goals for the Week
- Last week, had experiment with no current at all
- Hypothesized that cells were dead?
- Run test with 1 day vs. 2 day old Shewie to determine if there is an effect
Tuesday: July 22, 2008
- started mtrB and wt cultures
- mtrB culture to be used as 2-day culture on Thursday
- troubleshoot LabVIEW
- Resistances were measured to be different as expected in the past experiment
- Some as low as 160 ohms
- Perhaps the resistance of the chambers are less than we expected?
Wednesday: July 23, 2008
- Started another mtrB culture
- To be used as 1 day culture in experiment on Thursday
- LabVIEW
- Determined that resistance should be measured before connecting to the fuel cells
- Resistance of chambers is the same as what we measured initially
Thursday: July 24, 2008
- mtrB 1 day vs. 2 day experiment
- 6 chambers
- 3 chambers with 1-day old mtrB
- 3 chambers with 2-day old mtrB
- Injected lactate after the initial current spike came down
- Hypothesis
- 2-day cultures will have lower (if any) current production
- 6 chambers
Friday: July 25, 2008
mtrB: Overnight versus 2 Day Liquid Cultures
What we did:
-Ran 1-day old mtrB versus 2-day old mtrB
Results:
-The shapes of the curves were all very similar.
-Overnight cultures had slightly higher current production than 2-day old cultures.
