Team:Hawaii/Effect of CO2 on Growth Experiment

From 2008.igem.org

Birds Eye View Team	Projects	Events	Resources
Sponsors	Experiments	Milestones	Protocols
	Notebook (t)	Meetings (t)

CO2 Experiment

Hypothesis

SynechocystisPCC6803 is a cyanobacteria and therefore reduces CO₂ to carbohydrates. We hypothesize that if the incubator is equipped with CO₂gas flow, then colonies that grow will be more robust.

Methods

Four plates were streaked for colonies, two were placed in an incubator equipped with CO₂ flow while two were placed in an incubator lacking CO₂ flow.

Results

The two plates from the incubator equipped with CO₂ were observed today. There were more colonies and furthermore, the colonies were twice as big.

Conclusions

With our no-CO₂ incubator, it is possible to get colonies, but this process would be optimized by using the CO₂ incubator. Considering the short span of time we have to work in, and the already slow growth rate of Synechocystis PCC 6803, it would be to our advantage to grow our cultures in the presence of CO₂.

Things We Did Today

Prepared LB Broth

Margaret, Krystle, Norman

Created 2 500ml bottles of LB broth using Difco LB Broth, Miller

Discussion

If our action is not recorded, we are doomed to either repeat the error, or fail to replicate the success.

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]