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From 2008.igem.org

Birds Eye  View Team	Projects	Events	Resources
Sponsors	Experiments	Milestones	Protocols
	Notebook (t)	Meetings (t)

Contents 1 Agenda 2 Minutes 3 Action Items 4 Coming Up

Agenda

1. Team Agreement (Gernot)
2. Presentations:
   1. BioBricks "Other" (GK)
   2. Plasmid (MR/NW)
   3. Plan Part B
      1. Margaret: cyan red dual plasmid system
      2. Grace: lux operon night light
      3. Krystle: bacterial export system, soluble proteins for crystallagraphy
3. Team Photo
4. After 9-10am meeting, Jamie will show the lab safety, chemical storage, etc walkthrough.

Minutes

Present: GK, KS, MR, SC, GP, KS, NW

1. Presentations:
   1. File:Biobrick parts other.pdf (GK)
      1. Other Parts (GK): Construction Intermediates, PCR Primers, Tags & Modifiers, Other Other Parts, Deleted Parts, Bacteriophate T7 Phage Parts & RNA Stabilizers.
   2. Plasmid (MR/NW)
      1. RSF1010 Overview: OriT, tra, mob overview
      2. Proposed design, 4 BioBrick subcomponents
      3. RNA Stability questions (regions past coding seq, how far do we PCR past carboxy terminus for possible RNA stability features? Are there any?)
      4. Discussion regarding its usefulness (will be used by a lot of people)
   3. Plan Part B
      1. Margaret: cyan red dual plasmid system
      2. Grace: lux operon night light
      3. Krystle: bacterial export system, soluble proteins for crystallagraphy
2. Team Photo
3. Jamie showed us around the lab safety, talked about chemical storage, etc walkthrough.

Action Items

• MR write up formal proposal with details on synthetic plasmid
• E. coli grown and frozen, possible competent cell made
• get autoclave trained

Coming Up

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

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