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Team:Hawaii/Notebook/2008-09-15
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
PCR from 9/13
- Margaret
- Rep, oriV- correct size, so restriction digest with X,P of PCR product
- omega- correct size, but still has a ladder, so re-digest of plasmid prep with S, X
- oriT did not show up, but I found a re-digest from 7/24 that I will use for further cloning
- positive control did not work, not sure if negative control worked or if I did something wrong.
Plasmid prep
- Grace
- Inoculated TB+amp100 with:
- 3A: nir+rbs+slr1 #2, 3
- 3A: nir+rbs+pilA #6
- 3A: nir+rbs+slr1+GFPf #10
- 3A: nir+rbs+pilA+GFPf12 # 2, 6
- 3A: nir+rbs+pilA+GFPf13 # 4, 6, 8, 10
- Inoculated TB+sp100
- BB-pRL1383a
Sequencing
- Grace
- ExoSAP'd, determined DNA conc., prep'd for sequencing:
- 3A: nir+rbs+slr1 #2, 3
- 3A: nir+rbs+pilA #6
- 3A: nir+rbs+slr1+GFPf #10
- 3A: nir+rbs+pilA+GFPf12 # 2, 6
- 3A: nir+rbs+pilA+GFPf13 # 4, 6, 8, 10
- plac+rbs+slr1+GFPf #8
- Sent in samples from today and from last week for sequencing by CORE Hawaii
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
