Team:Hawaii/Notebook/2008-09- 2

From 2008.igem.org

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Things we did today

Wetlab work

Plasmid prep

Grace & Krystle
  • BB-pRL1383a
  • GFPf + tt colonies #8, 17, and 21

Colony PCR

Grace
File:090208colonyPCR.jpg
EtBr stained 2% agarose gel ran at 60V for 3 hours. Two microliters of PCR reaction were loaded into each well.
  • Colony PCR of 3A ligation transformants

PCR

Grace
  • J33207 with HindIII/BamHI primers

RE digest

Grace
  • J33207 and BB-pRL1383a with HindIII and BamHI

Made media plates

Grace
  • 1 sleeve of LB+sp2.5+sm2.5
  • 2 sleeves of LB+amp100

Construction of GFP + TT

Krystle
  • Ligated GFP (cut with SP) with tt (synthesized) using T4 ligase
negative control: re'd GFP ligated to self

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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