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Team:Hawaii/Notebook/2008-10-20
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Large scale plasmid prep of BB-pRL1383a (blue)
- Grace
PCR of J04430 (lac+rbs+GFP+tt+tt)
- Grace
- Will act as (-) control for GFP secretion in cyanos
Overnight RE digest
- Grace
- EcoRI and PstI digest of BBpRL1383a and J04430
Made LB+sp100 plates
- Grace
Prepped for sequencing
- Krystle
- Sent out correct-sized product to CORE for sequencing
- nir+rbs
- slr+gfpf
Construction of GFP secretion device
- Krystle
- Restriction digested additional PCR product
- nir+rbs with EcoRI and SpeI
Discussion
Quote of the Day
Garbage truck dripping E. coli... this has got to stop. - Roswell Mayor, http://www.youtube.com/watch?v=f7mEb-p4F44 (ok maybe we should start taking Amp + Kan + Sm + Sp before going to the bathroom)
