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Team:Hawaii/Notebook/2008-10-21
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Insertion of lac-GFP device into BB-pRL1383a
- Grace
- Ran RE digests on gel
- Bands were all incorrect size
- Reinoculated TB+sp100 for plasmid prep tomorrow
- PCR of J04430 from filter paper to verify part; used H/B primers
Team:Hawaii/Antibiotic test for BB-pRL1383a
- Grace
- Made LB+spvariable plates with 50 μl 20X X-gal
- Transformed 100 μl DH5α with 2 μl BB-pRL1383a (4 ng)
- Plated 50 μl cells + SOB on each LB+sp plate; plated 20 μl untransformed cells + SOB on each control plate
- Incubated at 37C for 2 days
Construction of GFP secretion device
- Krystle
- Gel purified overnight restriction digested nir+rbs
- Loaded all of restriction digest into a 2% gel, which appeared blank after running for 1.5 hours
- Re-PCR nir+rbs and slr+gfpf from plasmid prep
- Restriction digest
- nir+rbs with EcoRI and SpeI
- slr+gfpf with XbaI and PstI
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
