Team:Hawaii/Notebook/2008-10-25

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Things we did today

Wetlab work

Verification of plasmid preps

Grace

EtBr stained 4% agarose gel ran at 60V for 2 hours.

PCR'd plasmid preps from yesterday
RE digested all preps using EcoRI and PstI for 3 hours
Ran on gel

Inoculated 50ml TB+sp₁₀₀ with single colony BB1 for plasmid prep

Grace

Construction of BB-pRL1383a/K125820

Krystle

Restriction digest BB-pRL1383a with EcoRI and PstI
Dephosphorelated restriction product with SAP for 1 hour
Ligated cut/dephosphorelated product with gel purified K125820 using T4 ligase

Drylab Work

iGEM presentation

Grace

Worked on presentation

parts

Margaret

added parts to registry

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

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