Team:Minnesota/Notebook

From 2008.igem.org

The Minnesota iGEM '08 team consists of groups working on two different projects. One team is located primarily in Minneapolis and is designing a comparator for their project. The other team is based in St. Paul and is working on a time bomb.
Team Comparator
Control systems are an integral component of almost all aspects of life. Whether it is in industrial, biological, or chemical applications, controllers provide a way to keep systems functioning properly. A vital part of any control system is the comparator. This component compares a set point value and a measured value, and determines which is larger. It then sends the appropriate signal to the controller, which reacts to bring the system back to the set point. In typical applications, the controller equipment is electronic. However, our team set out to create a comparator using only genetic components. This comparator could potentially be used as part of a new, solely biological control system that could be used to treat many diseases afflicting humans, for example diabetes. This comparator could compare the blood sugar of a patient to what it should be, and send this result to a control system that could compensate, for example by changing the levels of insulin. In order to undertake this task, a system involving six genes was designed. For our system, the two inputs (one representing the set point and one representing the measured value) are IPTG and ATC. These inputs will activate the transcription of the LacI and TetR proteins, and set in motion the rest of the system to produce the outputs. Depending on the amounts of the two inducer molecules added to the system, either green fluorescent protein (GFP) or red fluorescent protein(RFP) will be produced. The actual design of the system can be seen below. In order to complete this project, a total of six genes will have to be cloned into plasmids, and two new BioBrick parts will be produced. One will be a TetR and p22-MNT dual-repressed promoter, and the other will be a LacI and lambdaphage cI dual-repressed promoter. We will also build mathematical models and conduct computer simulations that will help with the designs. This project will pave the way for other parts of a true genetic PID controller to be produced, which could be an exciting scientific development in the near future.	Team Comparator

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Notebook

June
M	T	W	T	F	S	S
						[http://2008.igem.org/Minnesota/1_June_2008 1]
[http://2008.igem.org/wiki/index.php?title=Minnesota/2_June_2008&action=edit 2]	[http://2008.igem.org/wiki/index.php?title=Minnesota/3_June_2008&action=edit 3]	[http://2008.igem.org/wiki/index.php?title=Minnesota/4_June_2008&action=edit 4]	[http://2008.igem.org/wiki/index.php?title=Minnesota/5_June_2008&action=edit 5]	[http://2008.igem.org/wiki/index.php?title=Minnesota/6_June_2008&action=edit 6]	[http://2008.igem.org/wiki/index.php?title=Minnesota/7_June_2008&action=edit 7]	[http://2008.igem.org/wiki/index.php?title=Minnesota/8_June_2008&action=edit 8]
[http://2008.igem.org/wiki/index.php?title=Minnesota/9_June_2008&action=edit 9]	[http://2008.igem.org/wiki/index.php?title=Minnesota/10_June_2008&action=edit 10]	[http://2008.igem.org/wiki/index.php?title=Minnesota/11_June_2008&action=edit 11]	[http://2008.igem.org/wiki/index.php?title=Minnesota/12_June_2008&action=edit 12]	[http://2008.igem.org/wiki/index.php?title=Minnesota/13_June_2008&action=edit 13]	[http://2008.igem.org/wiki/index.php?title=Minnesota/14_June_2008&action=edit 14]	[http://2008.igem.org/wiki/index.php?title=Minnesota/15_June_2008&action=edit 15]
[http://2008.igem.org/wiki/index.php?title=Minnesota/16_June_2008&action=edit 16]	[http://2008.igem.org/wiki/index.php?title=Minnesota/17_June_2008&action=edit 17]	[http://2008.igem.org/Minnesota/18_June_2008 18]	[http://2008.igem.org/Minnesota/19_June_2008 19]	[http://2008.igem.org/Minnesota/20_June_2008 20]	[http://2008.igem.org/wiki/index.php?title=Minnesota/21_June_2008&action=edit 21]	[http://2008.igem.org/wiki/index.php?title=Minnesota/22_June_2008&action=edit 22]
[http://2008.igem.org/Minnesota/23_June_2008 23]	[http://2008.igem.org/Minnesota/24_June_2008 24]	[http://2008.igem.org/Minnesota/25_June_2008 25]	[http://2008.igem.org/Minnesota/26_June_2008 26]	[http://2008.igem.org/Minnesota/27_June_2008 27]	[http://2008.igem.org/wiki/index.php?title=Minnesota/28_June_2008&action=edit 28]	[http://2008.igem.org/wiki/index.php?title=Minnesota/29_June_2008&action=edit 29]
[http://2008.igem.org/Minnesota/30_June_2008 30]

July
M	T	W	T	F	S	S
	[http://2008.igem.org/Minnesota/1_July_2008 1]	[http://2008.igem.org/Minnesota/2_July_2008 2]	[http://2008.igem.org/Minnesota/3_July_2008 3]	[http://2008.igem.org/Minnesota/4_July_2008 4]	[http://2008.igem.org/wiki/index.php?title=Minnesota/5_July_2008&action=edit 5]	[http://2008.igem.org/wiki/index.php?title=Minnesota/6_July_2008&action=edit 6]
[http://2008.igem.org/Minnesota/7_July_2008 7]	[http://2008.igem.org/Minnesota/8_July_2008 8]	[http://2008.igem.org/Minnesota/9_July_2008 9]	[http://2008.igem.org/Minnesota/10_July_2008 10]	[http://2008.igem.org/Minnesota/11_July_2008 11]	[http://2008.igem.org/wiki/index.php?title=Minnesota/12_July_2008&action=edit 12]	[http://2008.igem.org/wiki/index.php?title=Minnesota/13_July_2008&action=edit 13]
[http://2008.igem.org/Minnesota/14_July_2008 14]	[http://2008.igem.org/Minnesota/15_July_2008 15]	[http://2008.igem.org/Minnesota/16_July_2008 16]	[http://2008.igem.org/Minnesota/17_July_2008 17]	[http://2008.igem.org/Minnesota/18_July_2008 18]	[http://2008.igem.org/wiki/index.php?title=Minnesota/19_July_2008&action=edit 19]	[http://2008.igem.org/wiki/index.php?title=Minnesota/20_July_2008&action=edit 20]
[http://2008.igem.org/Minnesota/21_July_2008 21]	[http://2008.igem.org/Minnesota/22_July_2008 22]	[http://2008.igem.org/Minnesota/23_July_2008 23]	[http://2008.igem.org/Minnesota/24_July_2008 24]	[http://2008.igem.org/Minnesota/25_July_2008 25]	[http://2008.igem.org/Minnesota/26_July_2008 26]	[http://2008.igem.org/Minnesota/27_July_2008 27]
[http://2008.igem.org/Minnesota/28_July_2008 28]	[http://2008.igem.org/Minnesota/29_July_2008 29]	[http://2008.igem.org/Minnesota/30_July_2008 30]	[http://2008.igem.org/Minnesota/31_July_2008 31]

August
M	T	W	T	F	S	S
				[http://2008.igem.org/Minnesota/1_August_2008 1]	[http://2008.igem.org/wiki/index.php?title=Minnesota/2_August_2008&action=edit 2]	[http://2008.igem.org/wiki/index.php?title=Minnesota/3_August_2008&action=edit 3]
[http://2008.igem.org/Minnesota/4_August_2008 4]	[http://2008.igem.org/Minnesota/5_August_2008 5]	[http://2008.igem.org/Minnesota/6_August_2008 6]	[http://2008.igem.org/Minnesota/7_August_2008 7]	[http://2008.igem.org/Minnesota/8_August_2008 8]	[http://2008.igem.org/wiki/index.php?title=Minnesota/9_August_2008&action=edit 9]	[http://2008.igem.org/wiki/index.php?title=Minnesota/10_August_2008&action=edit 10]
[http://2008.igem.org/wiki/index.php?title=Minnesota/11_August_2008&action=edit 11]	[http://2008.igem.org/wiki/index.php?title=Minnesota/12_August_2008&action=edit 12]	[http://2008.igem.org/Minnesota/13_August_2008 13]	[http://2008.igem.org/wiki/index.php?title=Minnesota/14_August_2008&action=edit 14]	[http://2008.igem.org/wiki/index.php?title=Minnesota/15_August_2008&action=edit 15]	[http://2008.igem.org/wiki/index.php?title=Minnesota/16_August_2008&action=edit 16]	[http://2008.igem.org/wiki/index.php?title=Minnesota/17_August_2008&action=edit 17]
[http://2008.igem.org/wiki/index.php?title=Minnesota/18_August_2008&action=edit 18]	[http://2008.igem.org/wiki/index.php?title=Minnesota/19_August_2008&action=edit 19]	[http://2008.igem.org/wiki/index.php?title=Minnesota/20_August_2008&action=edit 20]	[http://2008.igem.org/wiki/index.php?title=Minnesota/21_August_2008&action=edit 21]	[http://2008.igem.org/wiki/index.php?title=Minnesota/22_August_2008&action=edit 22]	[http://2008.igem.org/wiki/index.php?title=Minnesota/23_August_2008&action=edit 23]	[http://2008.igem.org/wiki/index.php?title=Minnesota/24_August_2008&action=edit 24]
[http://2008.igem.org/wiki/index.php?title=Minnesota/25_August_2008&action=edit 25]	[http://2008.igem.org/wiki/index.php?title=Minnesota/26_August_2008&action=edit 26]	[http://2008.igem.org/wiki/index.php?title=Minnesota/27_August_2008&action=edit 27]	[http://2008.igem.org/wiki/index.php?title=Minnesota/28_August_2008&action=edit 28]	[http://2008.igem.org/wiki/index.php?title=Minnesota/29_August_2008&action=edit 29]	[http://2008.igem.org/wiki/index.php?title=Minnesota/30_August_2008&action=edit 30]	[http://2008.igem.org/wiki/index.php?title=Minnesota/31_August_2008&action=edit 31]