Team:NYMU-Taipei/Project/Phosphate/Experiments/Assembly

From 2008.igem.org

Contents

Auto-Regulated Phosphate Absorption Device

Phosphate Absorption Circuit

NYMU 20081007 pi absorption design.png

Phosphate Regulatory Circuit

NYMU 20081007 pi regulation design.png

Operations of Design

NYMU 20081007 pi operations.png

Testing Circuits

Test of pTet and tetR regulatory pair

NYMU 20081007 pi regulation test.png

  • RBS (B0034) + tetR (C0040) + D-term (B0015)
    • [http://partsregistry.org/Part:BBa_S03880 S03880] (failed;9/19, 2008)
    • [http://partsregistry.org/Part:BBa_P0440 P0440] (succeed; 9/19, 2008)
  • plasmid extraction (9/20)
    • pLac: R0010 (54.2 ng/uL)
    • pTet: R0040 (26.4 ng/uL)
    • RBS: B0032 (80.9 ng/uL)
    • D-term: B0015 (118.4 ng/uL)
  • plasmid extraction (9/23)
    • P0440 (114.62 ng/uL)
  • vector digestion (9/23)
    • pLac: R0010 D. SP (10.87 ng/uL)
    • pTet: R0040 D. SP (5.55 ng/uL)
    • RBS: B0032 D. SP (15.32 ng/uL)

Test of PPK and PST for pi absorption

NYMU 20081007 pi absorption test.png

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