Infection

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PRINCETON IGEM 2008

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Infection Protocol

Count cells.

Add 1x105 cells to each well.

Add 0.8 ul polybrene for each ml of media in the well.

Slide to mix.

Add virus – one tube (~15ul) to each well. (BLEACH everything used this point and later!)

Incubate 8-24 hours.

Change media (1mL per well).