Team:Rice University/Notebook/18 July 2008

From 2008.igem.org

Friday, July 18th

  • David Ouyang
    • Miniprep R0040 (ptet)
    • Digest Amber Suppressor and ptet
    • Ligate together
    • Do ARFP mutant quikchange and DPN1
    • Transform ARFP and Amber Suppressor supposed
  • Taylor Stevenson
  • Repeat PCR from yesterday using these primers (NOTE: to view primers download this link and change extension to .doc (word file)[1]).
    • tried raising annealing temperature to 58*C

20080718.jpg

    1. 100bp standard
    2. BleoC PCR product
    3. Stf PCR product
    4. Ligation of yesterdays digested and CIPed PCR primers
    5. 1kb standard
    • Result-The bands at 750bp and 2.5kbp correspond to the PCR products of BleoC_AvrII and Stf_NotI. These bands were cleaned, concentrated, and digested with NotI.
    • The BleoC_AvrII digest was treated with CIP.
    • Digested DNA was cleaned and concentrated, and ligated overnight at room temperature.





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