Team:Rice University/Notebook/4 June 2008

From 2008.igem.org

Wednesday 4 June

  • Selim Sheikh:
    • Prepared 2 digestion solutions:
      • lambda DNA with XhoI and HindIII
      • lambda DNA with HindIII only
  • Taylor Stevenson
    • Titered the phage stocks obtained from packaging the NEB lambda genome. See packaging manual [1].
    • Result-no colonies grew with a 1/200 (phage mixture:cell culture) was plated. Additionally, the more diluted phage mixture showed a titer of approximately 10^5 pfu/uL.

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