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Team:Warsaw/Calendar-Main/31 August 2008
From 2008.igem.org
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Blue-white screening and rifampicin test in
GM2163
Michał L., Ewa
0.5 ml of each liquid culture was plated on solid LB supplemented with 200 ug/ml rifampicin + Tetracycline
Plasmids were isolated from the rest of cultures.
Gel electrophoresis of isolated plasmids and gel-out of
pZC320
Gel-outed plasmids were transformed to Top10 cells