Team:Waterloo/Notebook/Protocols/Inoculation

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Inoculation Protocol

Materials
DNA Samples
Ampicillin (2µL/sample)
Kanamycin (2.5µL/sample)
Liquid LB Media (1 tube/sample)

Instructions
1. Label all tubes.
2. Do the following in the flow hood:
- Pipette the correct amount of antibiotic into the corresponding DNA tubes. 2 µL for ApR, 2.5 µL KmR.
- Dip the sterile stick into the DNA and into the corresponding media tube.
3. Incubate at 37 degrees celcius overnight on the agitator.

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