Team:Chiba/Calendar-Home/9 September 2008

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>[[Team:Chiba|Home]] | [[Team:Chiba/Notebook|Notebook]]
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[[Team:Chiba/Calendar-Home/8 September 2008|8 September 2008 <]]|[[Team:Chiba/Calendar-Home/10 September 2008|> 10 September 2008]]
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==Laboratory work==
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===Team:Input===
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no work
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===Team:Communication===
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(6/9)--->[[Team:Chiba/protocol/PCR|PCR]] of [http://partsregistry.org/Part:BBa_I9030 BBa_I9030]
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--->'''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]'''
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{|align="justify"
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|[[Image:Chiba-0909-3.JPG]]
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|
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:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<tr>
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<td width="257">Sample DNA</td>
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<td>60</td>
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</tr>
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<tr>
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<td>Loading Dye</td>
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<td>12</td>
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</tr>
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<tr>
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<td>TOTAL</td>
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<td>72μL</td>
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</tr>
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</table>
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::CinI
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|}
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--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]'''
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::*CinI
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::[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]
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::[http://partsregistry.org/Part:BBa_C0261 BBa_C0261]
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::[http://partsregistry.org/Part:BBa_S03154 BBa_S03154]
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::[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]
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--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
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{|align="justify"
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|[[Image:Chiba-0909.JPG]]
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|
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:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<tr>
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<td width="257">Sample DNA</td>
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<td>1</td>
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</tr>
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<tr>
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<td>Loading Dye</td>
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<td>1</td>
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</tr>
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<tr>
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<td>dH<sub>2</sub>O</td>
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<td>4</td>
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</tr>
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<tr>
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<td>TOTAL</td>
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<td>6μL</td>
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</tr>
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</table>
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:From left;
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::CinI -> ?
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::[http://partsregistry.org/Part:BBa_C0161 BBa_C0161] -> OK
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::[http://partsregistry.org/Part:BBa_C0261 BBa_C0261] -> OK
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::[http://partsregistry.org/Part:BBa_S03154 BBa_S03154] -> OK (low)
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::Marker
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::[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] -> OK (low)
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|}
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{|align="justify"
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|[[Image:Chiba-0909-2.JPG]]
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|
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:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<tr>
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<td width="257">Sample DNA</td>
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<td>1</td>
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</tr>
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<tr>
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<td>Loading Dye</td>
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<td>1</td>
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</tr>
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<tr>
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<td>dH<sub>2</sub>O</td>
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<td>4</td>
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</tr>
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<tr>
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<td>TOTAL</td>
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<td>6μL</td>
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</tr>
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</table>
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::[http://partsregistry.org/Part:BBa_J04500 BBa_J04500] -> OK
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|}
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#LuxI([http://partsregistry.org/Part:BBa_K084012 BBa_K084012])
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#LuxI+LVA([http://partsregistry.org/Part:BBa_K084014 BBa_K084014])
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#LasI([http://partsregistry.org/Part:BBa_K084007 BBa_K084007])
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#Background([http://partsregistry.org/Part:BBa_R0010 R0010])
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#Background([http://partsregistry.org/Part:BBa_C0261 BBa_C0261])
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:--->'''[[Team:Chiba/protocol/ligation/ligation|Ligation]]'''
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<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<tr>
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<td width="257">Sample No.</td>
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<td>(1)</td><td>(2)</td><td>(3)</td><td>(4)</td><td>(5)</td>
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</tr>
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<tr>
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<td>insert-C0161</td>
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<td>3</td><td>-</td><td>-</td><td>-</td><td>-</td>
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</tr>
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<tr>
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<td>insert-C0261</td>
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<td>-</td><td>2</td><td>-</td><td>-</td><td>2</td>
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</tr>
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<tr>
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<td>insert-S03154</td>
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<td>-</td><td>-</td><td>6</td><td>-</td><td>-</td>
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</tr>
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<tr>
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<td>Vector-R0010</td>
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<td>-</td><td>5</td><td>5</td><td>5</td><td>-</td>
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</tr>
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<tr>
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<td>vector-J04500</td>
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<td>3</td><td>-</td><td>-</td><td>-</td><td>-</td>
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</tr>
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<tr>
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<td>ligase</td>
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<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
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</tr>
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<tr>
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<td>Buffer</td>
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<td>2</td><td>2</td><td>4</td><td>2</td><td>2</td>
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</tr>
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<tr>
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<td>dH<sub>2</sub>O</td>
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<td>1</td><td>-</td><td>4</td><td>2</td><td>5</td>
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</tr>
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<tr>
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<td>TOTAL</td>
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<td>10</td><td>10</td><td>20</td><td>10</td><td>10</td>
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</tr>
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</table>
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<br clear=all>
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:---> '''[[Team:Chiba/protocol/transformation|Transformation]]'''
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---> '''Colony Count'''( 10/9)
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===Team:Output===
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no work

Latest revision as of 01:26, 30 October 2008

>Home | Notebook

8 September 2008 <|> 10 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

(6/9)--->PCR of [http://partsregistry.org/Part:BBa_I9030 BBa_I9030]


--->Gel Extract

Chiba-0909-3.JPG
Sample DNA 60
Loading Dye 12
TOTAL 72μL
CinI


--->Zymo Clean

  • CinI
[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]
[http://partsregistry.org/Part:BBa_C0261 BBa_C0261]
[http://partsregistry.org/Part:BBa_S03154 BBa_S03154]
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]


--->Gel Check

Chiba-0909.JPG
Sample DNA 1
Loading Dye 1
dH2O 4
TOTAL 6μL
From left;
CinI -> ?
[http://partsregistry.org/Part:BBa_C0161 BBa_C0161] -> OK
[http://partsregistry.org/Part:BBa_C0261 BBa_C0261] -> OK
[http://partsregistry.org/Part:BBa_S03154 BBa_S03154] -> OK (low)
Marker
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] -> OK (low)
Chiba-0909-2.JPG
Sample DNA 1
Loading Dye 1
dH2O 4
TOTAL 6μL
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500] -> OK
  1. LuxI([http://partsregistry.org/Part:BBa_K084012 BBa_K084012])
  2. LuxI+LVA([http://partsregistry.org/Part:BBa_K084014 BBa_K084014])
  3. LasI([http://partsregistry.org/Part:BBa_K084007 BBa_K084007])
  4. Background([http://partsregistry.org/Part:BBa_R0010 R0010])
  5. Background([http://partsregistry.org/Part:BBa_C0261 BBa_C0261])
--->Ligation
Sample No. (1)(2)(3)(4)(5)
insert-C0161 3----
insert-C0261 -2--2
insert-S03154 --6--
Vector-R0010 -555-
vector-J04500 3----
ligase 11111
Buffer 22422
dH2O 1-425
TOTAL 1010201010


---> Transformation

---> Colony Count( 10/9)

Team:Output

no work

Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/9_September_2008"