"
Team:KULeuven/10 September 2008
From 2008.igem.org
(Difference between revisions)
(→Wet Lab) |
m |
||
| (One intermediate revision not shown) | |||
| Line 1: | Line 1: | ||
| + | {{:Team:KULeuven/Tools/Styling}} | ||
{{:Team:KULeuven/Tools/Header}} | {{:Team:KULeuven/Tools/Header}} | ||
| + | |||
| + | {{:Team:KULeuven/Tools/New_Day/Date_Retriever}} | ||
== Lab Work == | == Lab Work == | ||
| Line 15: | Line 18: | ||
Blending the ethics... | Blending the ethics... | ||
| - | |||
| - | |||
| - | |||
| - | |||
== Remarks == | == Remarks == | ||
FC André had an important match today, the final score was 8-8! We were there and it was clear our team was far better than the others! They diserved to win!!!! | FC André had an important match today, the final score was 8-8! We were there and it was clear our team was far better than the others! They diserved to win!!!! | ||
| - | |||
| - | |||
Latest revision as of 01:45, 30 October 2008
dock/undock dropdown
| << return to notebook | return to homepage >> | ||
| < previous friday | ← yesterday | tomorrow → | next monday > |
Contents |
Lab Work
Wet Lab
- We MiniPrepped the ligations that succeeded yesterday: K145201+K145205, K145201+R0040+E0240, R0040+B0033+C0056+B0015. Then K145258 was cut with EcoRI and SpeI.
- We also made a glycerolstock of these ligations.
- The digests we made yesterday were put on gel. Then we extracted them and put again 2ul on gel to estimate the concentration.
- We ligated the following parts: K145201 + (R0040+P0353, C0062 (Pst) + (K145150+B0034, K145253 + K145254, (I712074+J23032) + (E0040+B0015), (B0014+B0033) + (K145151+B0015), B0015 (Pst) + (K145013+pSB1A2).
- We cut B0034 and J23109+J23032 with EcoRI and XbaI.
- We made a liquid culture of K145279 and K145280 to test the relation input/output with anhydrotetracycline.
Dry Lab
Blending the ethics...
Remarks
FC André had an important match today, the final score was 8-8! We were there and it was clear our team was far better than the others! They diserved to win!!!!
