Team:KULeuven/14 September 2008

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== Lab Work ==
== Lab Work ==
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* PCR to test electroporations of yesterday: We had colonies of J23116+B0034 and the PCR test showed that it was ligated properly.
* PCR to test electroporations of yesterday: We had colonies of J23116+B0034 and the PCR test showed that it was ligated properly.
* Ligation mix: K145014+pSB1A2, J23109+J23078, K145253+K145254, J23116+K145201, J23116+B0032+K145201 and (K145150+B0034)+C0062.
* Ligation mix: K145014+pSB1A2, J23109+J23078, K145253+K145254, J23116+K145201, J23116+B0032+K145201 and (K145150+B0034)+C0062.
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=== Dry Lab ===
 
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==== Modeling ====
 
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==== Wiki ====
 
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== Remarks ==
 
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Latest revision as of 01:47, 30 October 2008

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Lab Work

Wet Lab

We occupied ourself in the lab with the usual stuff:

  • Miniprep: K145257.
  • Electroporation: C0062+B0015, I712074+J23078 and K145275.
  • Digests: K145257 was cut with EcoRI and XbaI, yesterday's digests were continued:
    cut with Pst I -> J23109, K145150+B0034 and K145013.
    cut with EcoR I -> K145272, K145013+B0015, K145001+B0015, K145254 and K145201.
  • PCR to test electroporations of yesterday: We had colonies of J23116+B0034 and the PCR test showed that it was ligated properly.
  • Ligation mix: K145014+pSB1A2, J23109+J23078, K145253+K145254, J23116+K145201, J23116+B0032+K145201 and (K145150+B0034)+C0062.
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