Team:Paris/Modeling/f0

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[[Image:F0.jpg|thumb]] In the '''exponential phase of growth''', at the steady state, we have
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{{Paris/Menu}}
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{{Paris/Header|Method & Algorithm : ƒ0 }}
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<center> = act_J23101 </center>
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<br>
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[[Image:F0.jpg|thumb|Specific Plasmid Characterisation for &#131;0]]
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According to the characterization plasmid (see right) and to our modeling, in the '''exponential phase of growth''', at the steady state, the experiment would give us
[[Image:eqF0.jpg]]
[[Image:eqF0.jpg]]
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<div style="text-align: center">
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{{Paris/Toggle|Table of Values|Team:Paris/Modeling/More_f0}}
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</div>
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<center>That will directly give us &#131;0</center>
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{|border="1" style="text-align: center"
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<br>
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|param
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<br>
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|signification
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|unit
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|value
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|-
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|[exprJ23101]
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|expression rate of <br> J23101 '''with RBS E0032'''
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|nM.min<sup>-1</sup>
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|
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|-
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|γ<sub>GFP</sub>
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|dilution-degradation rate <br> of GFP(mut3b)
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|min<sup>-1</sup>
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|0.0198
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|-
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|[GFP]
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|GFP concentration at steady-state
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|nM
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|
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|-
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|(''fluorescence'')
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|value of the observed fluorescence
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|au
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|
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|-
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|''conversion''
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|conversion ratio between <br> fluorescence and concentration
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|nM.au<sup>-1</sup>
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|(1/79.429)
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|}
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That will directly give us &#131;0, which corresponds to &beta;<sub>72</sub> in the [[Team:Paris/Modeling/Synchronisation|equations]], if the ''pConstitituve'' is J23101.
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<center>
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[[Team:Paris/Modeling/Characterization_Approach| <Back - to "Implementation" ]]| <br>
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[[Team:Paris/Modeling/Characterization_Approach| <Back - to "Protocol_Of_Characterization" ]]|
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</center>

Latest revision as of 02:04, 30 October 2008

Method & Algorithm : ƒ0


= act_J23101


Specific Plasmid Characterisation for ƒ0

According to the characterization plasmid (see right) and to our modeling, in the exponential phase of growth, at the steady state, the experiment would give us

EqF0.jpg

↓ Table of Values ↑


param signification unit value comments
(fluorescence) value of the observed fluorescence au mean value of ~20 measures
conversion conversion ratio between
fluorescence and concentration
↓ gives ↓ 		nM.au-1 (1/79.429)
[GFP] GFP concentration at steady-state nM
γGFP dilution-degradation rate
of GFP(mut3b)
↓ gives ↓ 		min-1 0.0198 Only dilution :
Time Cell Division : 35 min.
ƒ0 activity of
J23101 with RBS E0032 		nM.min-1


That will directly give us ƒ0



<Back - to "Implementation" |
<Back - to "Protocol_Of_Characterization" |

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