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Team:UC Berkeley/GatewayPhagemid
From 2008.igem.org
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==Introduction== | ==Introduction== | ||
The phagemid scheme was motivated by a desire to further streamline the protocol for performing Gateway ''in vivo''. Like the genomic scheme, this method utilizes positive selection to isolate the desired product. However, it improves on this scheme because the lysis and infection steps replace mini-preps and transformations without requiring additional devices. | The phagemid scheme was motivated by a desire to further streamline the protocol for performing Gateway ''in vivo''. Like the genomic scheme, this method utilizes positive selection to isolate the desired product. However, it improves on this scheme because the lysis and infection steps replace mini-preps and transformations without requiring additional devices. | ||
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| + | This scheme utilizes two methods of positive selection: inducible origins of replication (''oriV'' and ''oriR6K'') and the ability to be packaged into and transported by a phage. The phagemid | ||
==Details about Plasmids and Strains== | ==Details about Plasmids and Strains== | ||
Revision as of 03:08, 30 October 2008
Phagemid Based Gateway
Introduction
The phagemid scheme was motivated by a desire to further streamline the protocol for performing Gateway in vivo. Like the genomic scheme, this method utilizes positive selection to isolate the desired product. However, it improves on this scheme because the lysis and infection steps replace mini-preps and transformations without requiring additional devices.
This scheme utilizes two methods of positive selection: inducible origins of replication (oriV and oriR6K) and the ability to be packaged into and transported by a phage. The phagemid
Details about Plasmids and Strains
General Procedure
Conclusion
