Team:Waterloo

From 2008.igem.org

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|The UW iGEM team is an interdisciplinary undergraduate-driven group spanning the faculties of Science, Mathematics, and Engineering. Our undergraduate members and graduate and faculty advisors bring skills, expertise, and perspectives from a broad range of fields, including Biology, Biochemistry, Computer Science, Bioinformatics, Computer and Electrical Engineering, Chemical Engineering, and Mathematical Physics.
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Our goal is to engineer a genome-free, cell-based expression system capable of producing a desired protein in response to environmental signals. The genome will be degraded by the combined activity of a restriction endonuclease (to fragment the genome) and an exonuclease (to hasten degradation of the genome). The gene for the protein of interest will be located on a plasmid which will lack recognition sites for the endonuclease, enabling it to remain intact after genome degradation. Expression of plasmid genes is expected to continue for a period of time until the "cell" expires.
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|[[Image:Example_logo.png|right|frame|Your team picture]]
 
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|align="center"|[[Team:Waterloo | Team Example 2]]
 
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<!--- The Mission, Experiments --->
 
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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| style="font-size:600%;  padding:50px; background-color:#FBCC30; width:100%; text-align:center" colspan="2" | '''University of Waterloo'''
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  |style="font-size:200%;  padding:15px; background-color:#FBCC30; width:100%; text-align:center" colspan="2" | '''Genome-free Bacterial Bioproduct Factory:'''
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A plasmid-safe, inducible genome-degradation strain for post-kill gene expression
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  |style="font-size:130%;  padding:5px; background-color:#FBCC30; width:100%; text-align:center" colspan="2" |
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'''''Code Name: The Headless Chicken Project'''''
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{| style="color:#1b2c8a;background-color:#FBCC30;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
!align="center"|[[Team:Waterloo|Home]]
!align="center"|[[Team:Waterloo|Home]]
!align="center"|[[Team:Waterloo/Team|The Team]]
!align="center"|[[Team:Waterloo/Team|The Team]]
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!align="center"|[[Team:Waterloo/Modeling|Modeling]]
!align="center"|[[Team:Waterloo/Modeling|Modeling]]
!align="center"|[[Team:Waterloo/Notebook|Notebook]]
!align="center"|[[Team:Waterloo/Notebook|Notebook]]
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!align="center"|[[Team:Waterloo/Sponsors|Sponsors]]
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    <td align="center">[[Image:UWiGEMLogo.png|200px|right]]</td>
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<td align="left"> The University of Waterloo 2008 iGEM Team is an interdisciplinary, undergraduate-run group spanning the Faculties of Science, Mathematics, and Engineering. Our undergraduate members and graduate and faculty advisors bring skills, expertise, and perspectives from a broad range of fields, including Biology, Biochemistry, Computer Science, Bioinformatics, Electrical and Computer Engineering, Chemical Engineering, and Mathematical Physics.
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| style="font-size:large; border-bottom-width:thick; border-bottom-color:black; border-bottom-style:solid; padding:5px; background-color:#FBCC30" colspan="2" | '''Genome-free Bacterial Bioproduct Factory:'''
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A plasmid-safe, inducible genome-degradation strain for post-kill gene expression
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'''''Code Name: The Headless Chicken Project'''''
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'''Abstract'''
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The aim of our project is to engineer a genome-free, cell-based expression system capable of producing a desired protein or activating a pathway in response to an environmental signal. Genome degradation is achieved using the combined activity of a restriction endonuclease to fragment the genome and an exonuclease to hasten degradation. The gene for the protein of interest will be located in a plasmid lacking recognition sites for the endonuclease, allowing it to remain intact after genome degradation. The plasmid genes will be expressed using the remaining cell resources until they expire. The primary application of this design would be an ''in situ'' compound production and delivery system for agricultural, industrial or therapeutic use to continue for a period of time.
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| style="font-size:large; border-bottom-width:thick; border-bottom-color:black; border-bottom-style:solid; padding:5px; background-color:#FBCC30" colspan="2" | [[Team:Waterloo/Sponsors|Gold Level Support]] Provided By
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| style="padding:20px; text-align:center"| <html><a href="http://www.mef.uwaterloo.ca/"><img src="https://static.igem.org/mediawiki/2008/3/32/MEF.PNG" width="300px" height="90px"><br>
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Mathematics Endowment Fund</a></html>
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| style="padding:20px"; text-align:center|<html><a href="http://www.science.uwaterloo.ca/"><img src="https://static.igem.org/mediawiki/2008/8/85/SciFac.PNG" width="300px" height="90px"><br>
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Faculty of Science</a></html>
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(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
 

Latest revision as of 04:00, 30 October 2008



University of Waterloo
Genome-free Bacterial Bioproduct Factory:

A plasmid-safe, inducible genome-degradation strain for post-kill gene expression

Code Name: The Headless Chicken Project


Home The Team The Project Parts Submitted to the Registry Modeling Notebook Sponsors
UWiGEMLogo.png
 The University of Waterloo 2008 iGEM Team is an interdisciplinary, undergraduate-run group spanning the Faculties of Science, Mathematics, and Engineering. Our undergraduate members and graduate and faculty advisors bring skills, expertise, and perspectives from a broad range of fields, including Biology, Biochemistry, Computer Science, Bioinformatics, Electrical and Computer Engineering, Chemical Engineering, and Mathematical Physics.


Genome-free Bacterial Bioproduct Factory:

A plasmid-safe, inducible genome-degradation strain for post-kill gene expression

Code Name: The Headless Chicken Project


Abstract

The aim of our project is to engineer a genome-free, cell-based expression system capable of producing a desired protein or activating a pathway in response to an environmental signal. Genome degradation is achieved using the combined activity of a restriction endonuclease to fragment the genome and an exonuclease to hasten degradation. The gene for the protein of interest will be located in a plasmid lacking recognition sites for the endonuclease, allowing it to remain intact after genome degradation. The plasmid genes will be expressed using the remaining cell resources until they expire. The primary application of this design would be an in situ compound production and delivery system for agricultural, industrial or therapeutic use to continue for a period of time.

WikiArt600.png


Gold Level Support Provided By

Mathematics Endowment Fund
Faculty of Science
Retrieved from "http://2008.igem.org/Team:Waterloo"