Team:Freiburg/Project

From 2008.igem.org

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<h2>'''Introduction:'''</h2>
+
<h2>'''Summary'''</h2>
-
This year´s main project is the attempt to create an "artificial receptor-system", featuring extra- and intracellular modules as well as suitable transmembrane regions.
+
'''Modular Synthetic Receptor System'''<br>
-
The intracellular domaine of our receptor-device is build by halves of split reporter-proteins that can reassemble and will then produce readable output, e. g. fluorescence.
+
[[Image:Freiburg08 MSRS Schema_txt.png|thumb|right|250 px]]
-
Each one of these protein-halves is connected to its extracellular domaine by a single-span transmembrane-helix.  
+
In the following we provide a summary of our project and display the highlights of our achievements. For a more detailed view please see the reports of each subproject.<br><br>
-
The extracellular or detecting domaine consists of a protein or peptide with the ability to bind a certain molecule.<br>
+
 
-
Now, if a system with two matching receptors is presented these molecules in a strict, pairwise spatial arrangement, the receptor-devices are brought together,
+
The general goal of the Freiburg 2008 team is to establish a synthetic transmembrane receptor system comprising the extracellular input devices, the extracellular receiver domain, the transducer to the cytosol and the reporter/executor in the cytosol.<br>  
-
the split reporter-protein reassembles inside the cell and the output can be detected.
+
As controllable receptor activation event, we chose spatial control of dimerization, which is a mechanism often used by nature. To achieve spatial control in the nanometer range we chose DNA origami for exact and complex patterns with several ligands and we opted for chemical coupling of one type of ligand to a scaffold protein for simple multimerization.<br>
-
We employ so-called "Origami-DNA" to create the exactly defined molecule-patterns that are needed to activate our receptors.
+
 
-
<br>
+
To facilitate binding, we used a single-chain Fv antibody fragment and a designed anticalin. Both bind haptens (nitro-jodo-phenol and fluorescein) which can be conjugated to specific oligonucleotides of the DNA origami or scaffold proteins. Transduction to the cytosol is mediated by a single transmembrane helix taken either from the EGF-receptor or B-cell-receptor.<br>
-
One of the main inspirations that lead to the idea of creating a synthetic receptor-like fusion protein is based on an immunologic study on the signaling pathway of the T-Cell-Receptor (TCR) that has been performed by Wolfgang Schamel at the Max-Planck-Institute for immunology, Freiburg[1].
+
 
-
In this study he used modified TCRs with Fab-Fragment-singlechains of Anti-NIP –Antibodies fused to their ß-domaines by a flexible linker that would present them on the cell´s surface.
+
As intracellular reporters we employ split proteins. Here, we used the split lactamase from the iGEM2007 Freiburg project as well as the split fluorescent proteins CFP/cerulean and YFP/venus. To provide steric flexibility and reach of our binding and reporting modules we interspersed linkers between each functional domain.<br>
-
This modification would allow to investigate the influence of receptor-clustering on the intensity of the cell-signaling. It could been shown that there is a relation between the clustering of the antigen and, thus, of the receptors by presenting various peptides with certain amounts and arrangements of NIP-molecules as stimulus.<br>
+
 
-
Anyway, this experiment was restricted by the one-dimensionality of the antigen-fused peptides; at this point, the Origami-DNA comes into play:
+
All parts submitted feature full BioBrick compatibility and in addition allow for the construction of fusion proteins due to extended Biobrick 3.0 (Freiburg) standard. Cloning was done in <i>E. coli</i>. However, we test our receptors in human or mouse cells/chassis. Thus, to direct our constructs to the cell surface we attached a eukaryotic signal peptide taken from hEGF-R to the N-terminus of each synthetic receptor. In addition to our designed synthetic receptors, we initially also employed an existing T-cell line.<br>
-
Paul Rothemund had discovered that it is possible to shape M13-Phage single-strand-DNA simply adding oligonucleotides that would work as „brackets“ when complementing the long single-strand. In this way, one can generate DNA-squares of a certain size with „nods“ at certain distances.<br>
+
 
-
One member of our team, Daniel Hautzinger, has recently finished his diploma-thesis on Origami-DNA and the possibilities of generating patterns on these square surfaces by modifying the Oligo-nucleotides that build up the nod-points.
+
To reach our goal within the short given time frame we started several subprojects in parallel. Our subprojects listed here are defined along these projects. Besides designing and cloning parts we spent quite some time on establishing and analyzing the biological experiments. Initially we tested interfacing of DNA origami with cells and the spatial control via DNA origami using the existing T-cell line expressing the scFv fused to a T-cell receptor. For T-cell receptor experiments we used Ca<sup>2+</sup> signaling as read out. Synthetic receptor activation was analyzed using the formation of fluorescent proteins or turnover of a fluorescent lactamase substrate. In our experiments we addressed the following questions:<br>
-
As the antigen NIP can as well be fused to these oligos, it was now possible to present strictly defined two-dimensional antigen-patterns to T-Cells carrying the modified receptors mentioned above.<br>
+
 
-
This, again, made us come up with the idea of a transmembrane-fusion-protein that could be spatially arranged from outside the cell by the pattern on the Origami-DNA-surface.<br>
+
* Can we design DNA origami to induce receptor multimerization?<br>
-
Of course, the first extracellular domaine we had in mind was the anti-NIP-singlechain Schamel had used with his receptors. The first intracellular domaines should consist of the split-lactamase-halfes we designed as parts for last year´s iGEM, as this enzyme´s activity can be regained by complementation of the halves and detected by a fluorescent substrate.
+
* Can we improve DNA origami assembly yield by varying the staple oligonucleotide to scaffold DNA ratio?<br>
-
Now, we were looking for a single-span-transmembrane-protein; as the domaines of the Epidermal-Growth-Factor Receptor are well known, we chose to employ it´s transmembrane-helix and the signal-peptide mediating the construct´s insertion into the membrane.<br>
+
* Can we find buffer conditions, which mediate cell viability and origami stability?<br>
-
Further modules we had in mind were an Anti-Fluorescein-singlechain and a fluorescein-binding variety of Lipocalin by Arne Skerra as extracellular „detectors“ as well as the complementing halves of each one of the split-fluorophores „Cerulean“ (cyan) and „Venus“ (yellow) as intracellular „reporters“. These split-fluorophores feature cross-compatibility  between the N- and C-terminal halves (green fluorescence), enabling us to generate three different „outputs“ (yellow, blue, green) with only two molecules (NIP, FluA) building up the „input-pattern“ on the Origami-DNA-surface. <br><br>
+
* Can we downsize a Ca<sup>2+</sup> release assay to fit the sample sizes of our precious DNA origami?<br>
-
<h2>'''Subprojects:'''</h2>
+
* Do our constructs express and then localize in the membrane of eukaryotic cells?<br>
-
[[Team:Freiburg/Modeling|Modeling]]<br>
+
* Do DNA origamis bind to cells?<br>
-
[[DNA-Origami|DNA-Origami]]<br>
+
* Can we detect synthetic receptor activation?<br>
-
[[Team:Freiburg_Cloning Strategy|Cloning Strategy]]<br>
+
 
-
[[Team:Freiburg_Cell Culture|Cell Culture]]<br>
+
In short, we successfully addressed the first six questions. Experiments to demonstrate synthetic receptor activation are ongoing.
-
[[Team:Freiburg_Transfection|Transfection]]<br>
+
 
-
[[Team:Freiburg_Calcium Imaging|Calcium Imaging]]
+
For our modeling analyses we constructed various sets of differential equations describing our synthetic receptors and predicted split protein activation behaviour.
-
<h2>'''Results:'''</h2>
+
 
-
All of our constructs (see "Parts") were cloned successfully using our extended pre- and suffix. Transfection of 293T-cells has also been shown to work for most of our fusion proteins; so far, we could even show that at least our constructs with lipocalin FluA as extracellular domaine are integrated into the membrane (Fig.1, details see [[Team:Freiburg Transfection|Transfection]]).<br>
+
The labs of Kristian Müller and Katja Arndt provided all basic technology and support to get started. For advanced analyses several labs of the University of Freiburg and the Max-Planck-Institute of Immunobiology granted access to their instrumentation (e.g. atomic force micoscopy, confocal microscopy, FACS).
-
[[Image:Freiburg2008_SP_LIPO_GGGS_TM_bla1_YFP_1.jpg|400px]][[Image:Freiburg2008_TV_CMV_YFP___CFP_loeslich.jpg|400px]]
+
 
-
'''Fig.1:''' left: Transfection of 293T-Cells with one of our membrane-localized constructs (Part Bba_K157032-YFP), <br> right: cytosolic YFP<br><br>
+
 
-
All transfections were carried out with part Bba_K157040, one of our composite parts consisting of the transfection vector Bba_J52017 and a CMV-promotor. Due to the limited time range we were not able to activate/dimerize any of the various possible "receptor"-pairs yet, but we are hoping to receive a positive result in that concern until the jamboree.<br>
+
<br><br>
-
<h2>'''Discussion:'''</h2>
+
<h2>'''Subprojects'''</h2>
-
<h2>'''Literature:'''</h2>
+
*[[Team:Freiburg/Modeling|Modeling]]<br>
 +
*[[Team:Freiburg/3D-Modeling|3D-Modeling]]<br>
 +
*[[DNA-Origami|DNA-Origami]]<br>
 +
*[[Team:Freiburg_Cloning Strategy|Cloning Strategy]]<br>
 +
*[[Team:Freiburg_Transfection and Synthetic Receptor|Transfection and Synthetic Receptor Activation]]<br>
 +
*[[Team:Freiburg_Calcium Imaging|Cell Stability, Ca<sup>2+</sup> Signaling and DNA-Origami-Binding]]<br><br>
 +
 
 +
<h2>'''Highlights'''</h2>
 +
 
 +
<table>
 +
<tr>
 +
<td>
 +
<h4>[[Team:Freiburg/Modeling|Modeling]]</h4>
 +
<!-- Dimerization of extracellular receptor domains is an important necessity for the functionality of our Modular Synthetic Receptor System. Presenting the system a stimulus in form of spatially arranged ligands (nitro-iodo-phenol or fluorescein molecules) results in dimerization and thus the corresponding intracellular parts such as the split lactamase halves or split fluorescent proteins complement to measureable output. -->
 +
 
 +
Receptor dimerization and activation was analyzed by introducing and discussing two receptor dimerization models: One T cell receptor model and one general receptor model. A proper model for the Modular Synthetic Receptor System was constructed. It consists of 10 reaction kinetic equations, 9 ordinary differential equations including 25 variable parameters. Matlab m-files are embedded for further inspection.
 +
</td>
 +
<td>
 +
[[image:Freiburg2008_MSRSdNN.png|thumb|right|200px|Split protein activity in time dependent on ligand amount]]<br>
 +
</td>
 +
</tr>
 +
</table>
 +
----
 +
<table>
 +
<tr>
 +
<td>
 +
[[Image:Team-Freiburg2008_Lipo_alpha_nCFP.png|thumb|left|250 px|Structural model of expression part Bba_K157037]]
 +
</td>
 +
<td>
 +
<h4>[[Team:Freiburg/3D-Modeling|3D-Modeling]]</h4>
 +
We have created various three-dimensional models of our constructs using Pymol and SwissPdbViewer. Pdb-files of the huge DNA-Origami molecule were created as adequate as possible using Nano-Engineer V1.0 and then edited in Pymol. These Models were used to plan the spatial arrangement and, thus, input-pattern of antigens at a nanometer scale.<br><br><br>
 +
</td>
 +
</tr>
 +
</table>
 +
----
 +
<table>
 +
<tr>
 +
<td>
 +
<h4>[[DNA-Origami|DNA-Origami]]</h4>
 +
The creation of DNA-Origami was one of the first sub-projects we engaged in. Structural planing of the input pattern on the Origami-surface and order of the modified oligo-nucleotides were the first issues, increasing of the Origami-yield and measurement of the molecule´s stability in cell culture media the later ones.<br>
 +
</td>
 +
<td>
 +
[[Image:Team Freiburg2008-Origami 1zu5.jpg|thumb|right|200 px|AFM-measurement of DNA-Origami]]
 +
</td>
 +
</tr>
 +
</table>
 +
----
 +
<table>
 +
<tr>
 +
<td>
 +
[[Image:Freiburg2008 Konstrukte.jpg|thumb|left|250px|Cloning scheme]]
 +
</td>
 +
<td>
 +
<h4>[[Team:Freiburg_Cloning Strategy|Cloning Strategy]]</h4>
 +
All of our constructs were cloned successfully using our extended pre- and suffix and plasmid "pMA" (part [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157000 Bba_K157000]). The broad combinatorial range given by the modular concept was almost fully exploited, so that we ended up with the submission of 13 basic and 28 composite parts.<br>
 +
Once a construct was completed, it was cloned into the CMV-promoted transfection vector (part [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157000 Bba_K157040]) and used for transfection of 293T-cells.<br>
 +
</td>
 +
</tr>
 +
</table>
 +
----
 +
<table>
 +
<tr>
 +
<td>
 +
<h4>[[Team:Freiburg_Transfection and Synthetic Receptor|Transfection and Synthetic Receptor Activation]]</h4>
 +
Transfection of 293T-cells has been shown to work for most of our fusion proteins; so far, we could even show that at least our constructs with lipocalin FluA as extracellular domaine are integrated into the membrane.<br>
 +
All transfections were carried out with part [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157040 Bba_K157040], one of our composite parts consisting of the transfection vector Bba_J52017 and a CMV-promotor. Due to the limited time range we were not yet able to demonstrate activation of the various possible "receptor"-pairs, but we are hoping to receive a positive result soon.<br>
 +
</td>
 +
<td>
 +
[[Image:Freiburg2008_Fluomembrane.jpg|thumb|right|200px|Membrane-localization of Part Bba_K157032-YFP]]
 +
</td>
 +
</tr>
 +
</table>
 +
----
 +
<table>
 +
<tr>
 +
<td>
 +
[[Image:TeamFreiburg2008_Pervanadate1.png|thumb|left|200px|Ca<sup>2+</sup>-influx measurement]]
 +
</td>
 +
<td>
 +
<h4>[[Team:Freiburg_Calcium Imaging|Cell Stability, Ca<sup>2+</sup> Signaling and DNA-Origami-Binding]]</h4>
 +
Due to the small total amounts of DNA-Origami we could not use FACS to measure calcium influx and, thus, T-cell activation. The alternative we found were "Nano-Slides" for inverse fluorescence microscopy; T-cells were immobilized on the poly-L-Lysine coated slides and stimulated under the microscope allowing for real-time observation of calcium-influx. Binding of fluorescent labeled DNA origami to cells was analyzed by confocal microscopy.<br>
 +
</td>
 +
</tr>
 +
</table>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
<h2>'''[[Image:MO2.jpg|50px|]]Literature'''</h2>
'''Split-fluorophores:'''<br>
'''Split-fluorophores:'''<br>
-
-Chang-Deng Hu, Yurii Chinenov, Tom K. Kerppola: ”Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation”, Molecular Cell, Vol. 9, 789–798, April, 2002<br>
+
*Chang-Deng Hu, Yurii Chinenov, Tom K. Kerppola: ”Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation”, Molecular Cell, Vol. 9, 789–798, April, 2002<br>
-
-Chang Deng Hu, Tom K. Kerppola: “Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis”, Nat Biotechnol. 2003 May; 21(5):539-545 (doi:10. 1038/nbt816)<br>
+
*Chang Deng Hu, Tom K. Kerppola: “Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis”, Nat Biotechnol. 2003 May; 21(5):539-545 (doi:10. 1038/nbt816)<br>
-
-Tom K. Kerppola: “Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells”, Nat Protoc. 2006;1(3):1278-1286 (doi:10.1038/nprot.2006.201)<br>
+
*Tom K. Kerppola: “Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells”, Nat Protoc. 2006;1(3):1278-1286 (doi:10.1038/nprot.2006.201)<br>
-
-Nagai, T. et al. “A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications” J. Biol. Chem. 276, 29188-29194, 2001<br>
+
*Nagai, T. et al. “A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications” J. Biol. Chem. 276, 29188-29194, 2001<br>
-
-Roger Y. Tsien et al. „Creating new fluorescent probes for cell biology“, Nature Biotechnology Reviews, Vol. 3, 906-918, 2002<br>
+
*Roger Y. Tsien et al. „Creating new fluorescent probes for cell biology“, Nature Biotechnology Reviews, Vol. 3, 906-918, 2002<br>
'''LipocalinFluA:'''<br>
'''LipocalinFluA:'''<br>
-
-Gerald Beste, Frank S. Schmidt, Thomas Stibora and A. Skerra: “Small antibody-like proteins with prescribed ligand specificities derived from the lipocalin fold“,Proc. Natl. Acad. Sci. USA Vol. 96, pp. 1898–1903, March 1999 Biochemistry<br>
+
*Gerald Beste, Frank S. Schmidt, Thomas Stibora and A. Skerra: “Small antibody-like proteins with prescribed ligand specificities derived from the lipocalin fold“,Proc. Natl. Acad. Sci. USA Vol. 96, pp. 1898–1903, March 1999 Biochemistry<br>
-
-Ingo P. Korndörfer, Gerald Beste and A. Skerra: “Crystallographic Analysis of an “Anticalin” With Tailored Specificity for Fluorescein Reveals High Structural Plasticity of the Lipocalin Loop Region”, PROTEINS: Structure, Function, and Bioinformatics 53:121–129 (2003)<br>
+
*Ingo P. Korndörfer, Gerald Beste and A. Skerra: “Crystallographic Analysis of an “Anticalin” With Tailored Specificity for Fluorescein Reveals High Structural Plasticity of the Lipocalin Loop Region”, PROTEINS: Structure, Function, and Bioinformatics 53:121–129 (2003)<br>
'''DNA-Origami:'''<br>
'''DNA-Origami:'''<br>
-
Paul W. K. Rothemund: Nature 440, 297-302 (16 March 2006)<br>
+
*Paul W. K. Rothemund: "Folding DNA to create nanoscale shapes and patterns", Nature 440, 297-302 (16 March 2006)<br>
'''Antibody B1-8:'''<br>
'''Antibody B1-8:'''<br>
-
-Ana Cumano and Klaus Rajewski: “Clonal recruitment and somatic mutation in the generation of immunological memory to the hapten NP”, The EMBO Journal vol. 5 no.10 pp. 2459-2468, 1986<br>
+
*Ana Cumano and Klaus Rajewski: “Clonal recruitment and somatic mutation in the generation of immunological memory to the hapten NP”, The EMBO Journal vol. 5 no.10 pp. 2459-2468, 1986<br>
-
-D. Allen, T. Simon, F. Sablitzky, K. Rajewski and A. Cumano: “Antibody engineering for the analysis of affinity maturation of an anti-hapten response”, The EMBO Journal vol. 7 no.7 pp. 1995-2001, 1988<br>
+
*D. Allen, T. Simon, F. Sablitzky, K. Rajewski and A. Cumano: “Antibody engineering for the analysis of affinity maturation of an anti-hapten response”, The EMBO Journal vol. 7 no.7 pp. 1995-2001, 1988<br>
 +
'''Modeling:'''<br>
 +
*Martin F. Bachmann, Michael Salzmann, Annette Oxenius and Pamela S. Ohashi: "Formation of TCR dimers/trimers as a crucial step for T cell activation", Eur. J. Immunol., 1998<br>
 +
*Martin F. Bachmann and Pamela S. Ohashi: "The role of T-cell receptor dimerization in T-cell activation", Review Immunology Today, 1999<br>
 +
*João Sousa and Jorge Carneiro: "A mathematical analysis of TCR serial triggering and down-regulation", Eur. J. Immunol., 2000<br>
 +
'''TCR activation:'''<br>
 +
*Susana Minguet, Mahima Swamy, Balbino Alarcón, Immanuel F. Luescher and Wolfgang W.A. Schamel: "Full Activation of the T Cell Receptor requires Both Clustering and Conformational Changes at CD3", Immunity, 2006
}}
}}

Latest revision as of 08:24, 30 October 2008


Freiburg2008 small header.gif



Home

The Team

Project Report

Parts

Modeling

Notebook

Safety

CoLABoration
_project report



Contents

Summary

Modular Synthetic Receptor System

Freiburg08 MSRS Schema txt.png

In the following we provide a summary of our project and display the highlights of our achievements. For a more detailed view please see the reports of each subproject.

The general goal of the Freiburg 2008 team is to establish a synthetic transmembrane receptor system comprising the extracellular input devices, the extracellular receiver domain, the transducer to the cytosol and the reporter/executor in the cytosol.
As controllable receptor activation event, we chose spatial control of dimerization, which is a mechanism often used by nature. To achieve spatial control in the nanometer range we chose DNA origami for exact and complex patterns with several ligands and we opted for chemical coupling of one type of ligand to a scaffold protein for simple multimerization.

To facilitate binding, we used a single-chain Fv antibody fragment and a designed anticalin. Both bind haptens (nitro-jodo-phenol and fluorescein) which can be conjugated to specific oligonucleotides of the DNA origami or scaffold proteins. Transduction to the cytosol is mediated by a single transmembrane helix taken either from the EGF-receptor or B-cell-receptor.

As intracellular reporters we employ split proteins. Here, we used the split lactamase from the iGEM2007 Freiburg project as well as the split fluorescent proteins CFP/cerulean and YFP/venus. To provide steric flexibility and reach of our binding and reporting modules we interspersed linkers between each functional domain.

All parts submitted feature full BioBrick compatibility and in addition allow for the construction of fusion proteins due to extended Biobrick 3.0 (Freiburg) standard. Cloning was done in E. coli. However, we test our receptors in human or mouse cells/chassis. Thus, to direct our constructs to the cell surface we attached a eukaryotic signal peptide taken from hEGF-R to the N-terminus of each synthetic receptor. In addition to our designed synthetic receptors, we initially also employed an existing T-cell line.

To reach our goal within the short given time frame we started several subprojects in parallel. Our subprojects listed here are defined along these projects. Besides designing and cloning parts we spent quite some time on establishing and analyzing the biological experiments. Initially we tested interfacing of DNA origami with cells and the spatial control via DNA origami using the existing T-cell line expressing the scFv fused to a T-cell receptor. For T-cell receptor experiments we used Ca2+ signaling as read out. Synthetic receptor activation was analyzed using the formation of fluorescent proteins or turnover of a fluorescent lactamase substrate. In our experiments we addressed the following questions:

  • Can we design DNA origami to induce receptor multimerization?
  • Can we improve DNA origami assembly yield by varying the staple oligonucleotide to scaffold DNA ratio?
  • Can we find buffer conditions, which mediate cell viability and origami stability?
  • Can we downsize a Ca2+ release assay to fit the sample sizes of our precious DNA origami?
  • Do our constructs express and then localize in the membrane of eukaryotic cells?
  • Do DNA origamis bind to cells?
  • Can we detect synthetic receptor activation?

In short, we successfully addressed the first six questions. Experiments to demonstrate synthetic receptor activation are ongoing.

For our modeling analyses we constructed various sets of differential equations describing our synthetic receptors and predicted split protein activation behaviour.

The labs of Kristian Müller and Katja Arndt provided all basic technology and support to get started. For advanced analyses several labs of the University of Freiburg and the Max-Planck-Institute of Immunobiology granted access to their instrumentation (e.g. atomic force micoscopy, confocal microscopy, FACS).




Subprojects

Highlights

Modeling

Receptor dimerization and activation was analyzed by introducing and discussing two receptor dimerization models: One T cell receptor model and one general receptor model. A proper model for the Modular Synthetic Receptor System was constructed. It consists of 10 reaction kinetic equations, 9 ordinary differential equations including 25 variable parameters. Matlab m-files are embedded for further inspection.

Split protein activity in time dependent on ligand amount
Structural model of expression part Bba_K157037

3D-Modeling

We have created various three-dimensional models of our constructs using Pymol and SwissPdbViewer. Pdb-files of the huge DNA-Origami molecule were created as adequate as possible using Nano-Engineer V1.0 and then edited in Pymol. These Models were used to plan the spatial arrangement and, thus, input-pattern of antigens at a nanometer scale.

DNA-Origami

The creation of DNA-Origami was one of the first sub-projects we engaged in. Structural planing of the input pattern on the Origami-surface and order of the modified oligo-nucleotides were the first issues, increasing of the Origami-yield and measurement of the molecule´s stability in cell culture media the later ones.
AFM-measurement of DNA-Origami
Cloning scheme

Cloning Strategy

All of our constructs were cloned successfully using our extended pre- and suffix and plasmid "pMA" (part [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157000 Bba_K157000]). The broad combinatorial range given by the modular concept was almost fully exploited, so that we ended up with the submission of 13 basic and 28 composite parts.
Once a construct was completed, it was cloned into the CMV-promoted transfection vector (part [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157000 Bba_K157040]) and used for transfection of 293T-cells.

Transfection and Synthetic Receptor Activation

Transfection of 293T-cells has been shown to work for most of our fusion proteins; so far, we could even show that at least our constructs with lipocalin FluA as extracellular domaine are integrated into the membrane.
All transfections were carried out with part [http://partsregistry.org/wiki/index.php?title=Part:BBa_K157040 Bba_K157040], one of our composite parts consisting of the transfection vector Bba_J52017 and a CMV-promotor. Due to the limited time range we were not yet able to demonstrate activation of the various possible "receptor"-pairs, but we are hoping to receive a positive result soon.
Membrane-localization of Part Bba_K157032-YFP
Ca2+-influx measurement

Cell Stability, Ca2+ Signaling and DNA-Origami-Binding

Due to the small total amounts of DNA-Origami we could not use FACS to measure calcium influx and, thus, T-cell activation. The alternative we found were "Nano-Slides" for inverse fluorescence microscopy; T-cells were immobilized on the poly-L-Lysine coated slides and stimulated under the microscope allowing for real-time observation of calcium-influx. Binding of fluorescent labeled DNA origami to cells was analyzed by confocal microscopy.



MO2.jpgLiterature

Split-fluorophores:

  • Chang-Deng Hu, Yurii Chinenov, Tom K. Kerppola: ”Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation”, Molecular Cell, Vol. 9, 789–798, April, 2002
  • Chang Deng Hu, Tom K. Kerppola: “Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis”, Nat Biotechnol. 2003 May; 21(5):539-545 (doi:10. 1038/nbt816)
  • Tom K. Kerppola: “Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells”, Nat Protoc. 2006;1(3):1278-1286 (doi:10.1038/nprot.2006.201)
  • Nagai, T. et al. “A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications” J. Biol. Chem. 276, 29188-29194, 2001
  • Roger Y. Tsien et al. „Creating new fluorescent probes for cell biology“, Nature Biotechnology Reviews, Vol. 3, 906-918, 2002

LipocalinFluA:

  • Gerald Beste, Frank S. Schmidt, Thomas Stibora and A. Skerra: “Small antibody-like proteins with prescribed ligand specificities derived from the lipocalin fold“,Proc. Natl. Acad. Sci. USA Vol. 96, pp. 1898–1903, March 1999 Biochemistry
  • Ingo P. Korndörfer, Gerald Beste and A. Skerra: “Crystallographic Analysis of an “Anticalin” With Tailored Specificity for Fluorescein Reveals High Structural Plasticity of the Lipocalin Loop Region”, PROTEINS: Structure, Function, and Bioinformatics 53:121–129 (2003)

DNA-Origami:

  • Paul W. K. Rothemund: "Folding DNA to create nanoscale shapes and patterns", Nature 440, 297-302 (16 March 2006)

Antibody B1-8:

  • Ana Cumano and Klaus Rajewski: “Clonal recruitment and somatic mutation in the generation of immunological memory to the hapten NP”, The EMBO Journal vol. 5 no.10 pp. 2459-2468, 1986
  • D. Allen, T. Simon, F. Sablitzky, K. Rajewski and A. Cumano: “Antibody engineering for the analysis of affinity maturation of an anti-hapten response”, The EMBO Journal vol. 7 no.7 pp. 1995-2001, 1988

Modeling:

  • Martin F. Bachmann, Michael Salzmann, Annette Oxenius and Pamela S. Ohashi: "Formation of TCR dimers/trimers as a crucial step for T cell activation", Eur. J. Immunol., 1998
  • Martin F. Bachmann and Pamela S. Ohashi: "The role of T-cell receptor dimerization in T-cell activation", Review Immunology Today, 1999
  • João Sousa and Jorge Carneiro: "A mathematical analysis of TCR serial triggering and down-regulation", Eur. J. Immunol., 2000

TCR activation:

  • Susana Minguet, Mahima Swamy, Balbino Alarcón, Immanuel F. Luescher and Wolfgang W.A. Schamel: "Full Activation of the T Cell Receptor requires Both Clustering and Conformational Changes at CD3", Immunity, 2006

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