Revision as of 20:37, 16 July 2008

Thursday 5 June

Selim Sheikh:
- Prepared gel electrophoresis of digestions from the previous day with 1Kb standard

(from right to left)
lane 1: 1Kb standard
lane 2: NEB lambda with HindIII
lane 3: NEB lambda with XhoI/HindIII)

Taylor Stevenson
- XL1-Blue MR cells were prepared for phage infection as specified in the [1] and infected with phage at roughly a 1/10 pfu/cfu ratio. Resulting phage/bacteria mixture was incubated @ 37*C for 20 min and then streaked onto an LB plate. Plate was incubated @ 30*C O/N.
- Result-approximately 100 possibly lysogenic colonies grew on plate.

Home	The Team	The Project	Parts Submitted to the Registry	Modeling	Notebook

@@ Line 9: / Line 9: @@
 <BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR>
 *Taylor Stevenson
-**XL1-Blue MR cells were prepared for phage infection as specified in the [[https://static.igem.org/mediawiki/2008/6/6a/Packaging_Extract.pdf]] and infected with phage at roughly a 1/10 pfu/cfu ratio.  Resulting phage/bacteria mixture was incubated @ 37*C for 20 min and then streaked onto an LB plate.  Plate was incubated @ 30*C O/N.
+**XL1-Blue MR cells were prepared for phage infection as specified in the [https://static.igem.org/mediawiki/2008/6/6a/Packaging_Extract.pdf] and infected with phage at roughly a 1/10 pfu/cfu ratio.  Resulting phage/bacteria mixture was incubated @ 37*C for 20 min and then streaked onto an LB plate.  Plate was incubated @ 30*C O/N.
 **'''Result'''-approximately 100 possibly lysogenic colonies grew on plate.
 <BR><BR><BR><BR>