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Team:Rice University/Notebook/5 June 2008
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=Thursday 5 June= | =Thursday 5 June= | ||
*Selim Sheikh: | *Selim Sheikh: | ||
| - | ** | + | **Prepared gel electrophoresis of digestions from the previous day with 1Kb standard |
[[Image:20080604_gel.jpg|100px|left]] | [[Image:20080604_gel.jpg|100px|left]] | ||
| - | (from | + | (from right to left) |
| + | <BR>lane 1: 1Kb standard | ||
| + | <BR>lane 2: NEB lambda with HindIII | ||
| + | <BR>lane 3: NEB lambda with XhoI/HindIII) | ||
| + | <BR><BR><BR><BR><BR><BR><BR><BR><BR><BR><BR> | ||
| + | *Taylor Stevenson | ||
| + | **XL1-Blue MR cells were prepared for phage infection as specified in the phage packaging manual [https://static.igem.org/mediawiki/2008/6/6a/Packaging_Extract.pdf] and infected with phage at roughly a 1/10 pfu/cfu ratio. Resulting phage/bacteria mixture was incubated @ 37*C for 20 min and then streaked onto an LB plate. Plate was incubated @ 30*C O/N. | ||
| + | **'''Result'''-approximately 100 possibly lysogenic colonies grew on plate. | ||
| + | <BR><BR><BR><BR> | ||
| + | {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" | ||
| + | !align="center"|[[Team:Rice_University|Home]] | ||
| + | !align="center"|[[Team:Rice_University/Team|The Team]] | ||
| + | !align="center"|[[Team:Rice_University/Project|The Project]] | ||
| + | !align="center"|[[Team:Rice_University/Parts|Parts Submitted to the Registry]] | ||
| + | !align="center"|[[Team:Rice_University/Modeling|Modeling]] | ||
| + | !align="center"|[[Team:Rice_University/Notebook|Notebook]] | ||
| + | |} | ||
Latest revision as of 20:38, 16 July 2008
Thursday 5 June
- Selim Sheikh:
- Prepared gel electrophoresis of digestions from the previous day with 1Kb standard
(from right to left)
lane 1: 1Kb standard
lane 2: NEB lambda with HindIII
lane 3: NEB lambda with XhoI/HindIII)
- Taylor Stevenson
- XL1-Blue MR cells were prepared for phage infection as specified in the phage packaging manual [1] and infected with phage at roughly a 1/10 pfu/cfu ratio. Resulting phage/bacteria mixture was incubated @ 37*C for 20 min and then streaked onto an LB plate. Plate was incubated @ 30*C O/N.
- Result-approximately 100 possibly lysogenic colonies grew on plate.
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