Latest revision as of 20:38, 16 July 2008

Thursday 5 June

Selim Sheikh:
- Prepared gel electrophoresis of digestions from the previous day with 1Kb standard

(from right to left)
lane 1: 1Kb standard
lane 2: NEB lambda with HindIII
lane 3: NEB lambda with XhoI/HindIII)

Taylor Stevenson
- XL1-Blue MR cells were prepared for phage infection as specified in the phage packaging manual [1] and infected with phage at roughly a 1/10 pfu/cfu ratio. Resulting phage/bacteria mixture was incubated @ 37*C for 20 min and then streaked onto an LB plate. Plate was incubated @ 30*C O/N.
- Result-approximately 100 possibly lysogenic colonies grew on plate.

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 *Taylor Stevenson
-**Titered the phage stocks obtained from packaging the NEB lambda genome
+**XL1-Blue MR cells were prepared for phage infection as specified in the phage packaging manual [https://static.igem.org/mediawiki/2008/6/6a/Packaging_Extract.pdf] and infected with phage at roughly a 1/10 pfu/cfu ratio.  Resulting phage/bacteria mixture was incubated @ 37*C for 20 min and then streaked onto an LB plate.  Plate was incubated @ 30*C O/N.
-*#Grow VCS275 supA+ strain in Lambda media (LB supplemented with 0.2% w/v maltose and 10mM MgS04) @ 30*C O/N, shaking @ 250rpm.
+**'''Result'''-approximately 100 possibly lysogenic colonies grew on plate.
-*#Pellet cells @ 500xg for 10min, and re-suspend in 2x volume of 10mM MgS04.  Aliquot into 200uL fractions.
+<BR><BR><BR><BR>
-*#Add serial dilutions of phage in SM buffer see ([packaging manual.pdf])
+{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+!align="center"|[[Team:Rice_University|Home]]
+!align="center"|[[Team:Rice_University/Team|The Team]]
+!align="center"|[[Team:Rice_University/Project|The Project]]
+!align="center"|[[Team:Rice_University/Parts|Parts Submitted to the Registry]]
+!align="center"|[[Team:Rice_University/Modeling|Modeling]]
+!align="center"|[[Team:Rice_University/Notebook|Notebook]]
+|}