Latest revision as of 20:38, 16 July 2008

Thursday 5 June

Selim Sheikh:
- Prepared gel electrophoresis of digestions from the previous day with 1Kb standard

(from right to left)
lane 1: 1Kb standard
lane 2: NEB lambda with HindIII
lane 3: NEB lambda with XhoI/HindIII)

Taylor Stevenson
- XL1-Blue MR cells were prepared for phage infection as specified in the phage packaging manual [1] and infected with phage at roughly a 1/10 pfu/cfu ratio. Resulting phage/bacteria mixture was incubated @ 37*C for 20 min and then streaked onto an LB plate. Plate was incubated @ 30*C O/N.
- Result-approximately 100 possibly lysogenic colonies grew on plate.

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 *Taylor Stevenson
-**Titered the phage stocks obtained from packaging the NEB lambda genome. See [[packaging manual.pdf]].
+**XL1-Blue MR cells were prepared for phage infection as specified in the phage packaging manual [https://static.igem.org/mediawiki/2008/6/6a/Packaging_Extract.pdf] and infected with phage at roughly a 1/10 pfu/cfu ratio.  Resulting phage/bacteria mixture was incubated @ 37*C for 20 min and then streaked onto an LB plate.  Plate was incubated @ 30*C O/N.
-**'''Result'''-no colonies grew with a 1/200 (phage mixture:cell culture) was plated.  Additionally, the more diluted phage mixture showed a titer of approximately 10^5 pfu/uL.
+**'''Result'''-approximately 100 possibly lysogenic colonies grew on plate.
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