"
Team:UNIPV-Pavia/Notebook/Week8
From 2008.igem.org
(Difference between revisions)
| Line 60: | Line 60: | ||
**No true positives for BBa_J23100-'''BBa_B0030'''-BBa_C0012 | **No true positives for BBa_J23100-'''BBa_B0030'''-BBa_C0012 | ||
**No true positives for BBa_J23100-'''BBa_B0030'''-BBa_I15010 | **No true positives for BBa_J23100-'''BBa_B0030'''-BBa_I15010 | ||
| + | **Non pure true positives for BBa_B0030-BBa_E0040-'''BBa_B1006''' | ||
**Pure true positives for BBa_B0030-BBa_C0051-'''BBa_B1006''' | **Pure true positives for BBa_B0030-BBa_C0051-'''BBa_B1006''' | ||
| - | ** | + | **Non pure true positives for BBa_B0030-BBa_E1010-'''BBa_B1006''' |
| + | |||
| + | *We chose to keep the first colony for all the 3 working ligation plates. | ||
| + | |||
| + | *NOTE: BBa_B0030-BBa_E0040-'''BBa_B1006''' and BBa_B0030-BBa_E1010-'''BBa_B1006''' are final parts; we decided to sequence these 2 parts even if gel showed a weak contamination: sequencing results will tell us if there are false positive plasmids in those colonies or if the contamination was only a PCR contamination. | ||
| + | |||
| + | *We infected 9 ml LB + suitable antibiotic with 30 | ||
Revision as of 10:24, 20 July 2008
 Home
|  The Team
|  The Project
|  Biological Safety
|  Parts Submitted to the Registry
|
|---|---|---|---|---|
 Dry Lab
|  Wet Lab
|  Modeling
|  Protocols
|  Activity Notebook
|
Notebook
| Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 |
|---|---|---|---|---|---|---|
| Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 |
Week 8: 07/7/08 - 07/12/08
07/7/08
- Colony PCR (5 colonies for each plate) for:
- BBa_J23100-BBa_B0030-BBa_C0012 (for the second time, we hoped to find true positive colonies)
- BBa_J23100-BBa_B0030-BBa_I15010 (for the second time, we hoped to find true positive colonies)
- BBa_B0030-BBa_E0040-BBa_B1006
- BBa_B0030-BBa_C0051-BBa_B1006
- BBa_B0030-BBa_E1010-BBa_B1006
 |
- Gel results:
- No true positives for BBa_J23100-BBa_B0030-BBa_C0012
- No true positives for BBa_J23100-BBa_B0030-BBa_I15010
- Non pure true positives for BBa_B0030-BBa_E0040-BBa_B1006
- Pure true positives for BBa_B0030-BBa_C0051-BBa_B1006
- Non pure true positives for BBa_B0030-BBa_E1010-BBa_B1006
- We chose to keep the first colony for all the 3 working ligation plates.
- NOTE: BBa_B0030-BBa_E0040-BBa_B1006 and BBa_B0030-BBa_E1010-BBa_B1006 are final parts; we decided to sequence these 2 parts even if gel showed a weak contamination: sequencing results will tell us if there are false positive plasmids in those colonies or if the contamination was only a PCR contamination.
- We infected 9 ml LB + suitable antibiotic with 30
