Team:UNIPV-Pavia/Notebook/Week9

(Difference between revisions)

Revision as of 14:03, 20 July 2008

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07/14/08

We transformed 1 µl of the 6 ligations stored at -20°C. We plated transformed bacteria and incubated plates at 37°C overnight.

BBa_R0079 (E-X)

BBa_B0030-BBa_C0078-BBa_B1006 (E-S)

07/15/08

We transformed 1 µl of BBa_B0030-BBa_C0078-BBa_B1006-BBa_R0079 ligation and plated transformed bacteria. We incubated the plate at 37°C overnight.

All the 6 overnight plates showed colonies. We performed colony PCR for (we decided to pick up more colonies for the ligations with long inserts):

Picked up colonies in LB + Amp

@@ Line 61: / Line 61: @@
 <br><br>
-'''07/14/08'''
+'''07/15/08'''
 <br>
 *We transformed 1 µl of BBa_B0030-BBa_C0078-BBa_B1006-'''BBa_R0079''' ligation and plated transformed bacteria. We incubated the plate at 37°C overnight.
-*All the 6 overnight plates showed colonies. We performed colony PCR for:
+*All the 6 overnight plates showed colonies. We performed colony PCR for (we decided to pick up more colonies for the ligations with long inserts):
-**
+#BBa_J23100-'''BBa_B0030''' (S-P) -BBa_C0012 (standard ligation) - 10 colonies
+#'''pSB1AK3'''-BBa_J23100-BBa_B0030-BBa_C0012 (double ligation) - 7 colonies
+#BBa_J23100-BBa_B0030-BBa_C0040-BBa_B1006-'''BBa_R0040''' - 3 colonies
+#BBa_R0051-BBa_B0030-BBa_C0062-BBa_B1006-'''BBa_R0062''' - 3 colonies
+#BBa_B0030-BBa_C0051-BBa_B0030-BBa_C0079-'''BBa_B1006''' - 10 colonies
+#BBa_B0030-BBa_I15009-'''BBa_B1006''' - 3 colonies
+{|
+|[[Image:pv_colonypcr_15_7_08.jpg|thumb|300px|left|Picked up colonies in LB + Amp]]
+|}
+{|
+|[[Image:pv_pcr_.jpg|thumb|300px|left|]]
+|}